间歇性负重游泳训练对大鼠杏仁基底外侧核(BLA)c-fos蛋白表达的影响

The Influence of Intermittent Swimming Exercise on the Expression of C-fos Protein in Basolateral Nucleus of Rat Amygdale

目的:观察间歇性负重游泳训练后大鼠杏仁基底外侧核(BLA)内c-fos蛋白表达的变化,探讨间歇性游泳训练对c-fos蛋白表达的诱导机制。方法:建立大鼠间歇性负重游泳训练模型,用ABC免疫组织化学方法检测训练后即刻、0.51、h2、h4、h大鼠BLA内c-fos蛋白表达状况,并通过图像分析系统和统计学软件进行图像和数据分析。结果:对照组大鼠BLA内c-fos阳性神经元少量散在分布;模型组明显增多,且随着时间的增长呈上升趋势(0.5 h<1 h<2 h),2 h达到峰值,与对照组相比具有非常显著性差异(P<0.01);然后逐渐下降。结论:间歇性负重游泳训练可引起大鼠BLA中c-fos蛋白表达上调,且具有一定的时效性,其机制可能与间歇运动能引起神经细胞内钙离子浓度变化有关。

Objective To investigate the mechanism of the expression of c-fos protein by observing the changing tendency of c-fos protein in basolateral nucleus of rat amygdale （BLA） after intermittent swimming exercise. Methods： To establish the intermittent swimming exercise models, and detect the expression of c-fos protein in BLA at the moments of 0 h, 0.5 h, 1 h, 2 h, 4 h after exercise by immunohistochemical SABC method, then analyze the image and data by image analyzing system and statistic software. Results： Several Fos ＋ neurons of control group distribute in the BLA dispersively while the swimming groups express significantly higher, and increases progressively with time （0.5 h 〈 1 h 〈 2 h）, the peak of Fos ＋ neurons happened at 2 h, and appears significant difference as compared with the control group （ P 〈 0. 01 ） ; then descends regularly. Conclusion： Intermittent swimming exercise raises the expression of c-fos protein in BLA, and has a certain timeliness. The mechanism has deal with the change of the intracellular calcium concentration at nerve cells.