白细胞介素-1、-6对鼠甲状腺FRTL-5细胞间通讯的影响

EFFECT OF INTERLEUKIN1, INTERLEUKIN6 ON THE INTERCELLULAR COMMUNICATION OF RAT THYROID FRTL5 CELLS

为探讨细胞因子对细胞间通讯的影响，应用荧光光淬灭后恢复技术研究白细胞介素（ＩＬ）－１、ＩＬ－６及促甲状腺激素（ＴＳＨ）对鼠甲状腺ＦＲＴＬ－５细胞与细胞间通讯的作用。将培养的ＦＲＴＬ－５细胞加入不同浓度的ＴＳＨ、ＩＬ－１β、ＩＬ－６继续培养１２小时后作荧光染色，以激光扫描仪测定平均荧光恢复速率（ＭＦＲＲ）作为通讯程度的定量指标。结果：（１）用ＴＳＨ后细胞ＭＦＲＲ（单位：％／ｍｉｎ）对照组为０．４４５±０．０３３，０．１、１、５Ｕ／Ｌ组分别为０．６７９±０．０５４、０．９５０±０．０７３、０．７９９±０．０８２（Ｐ＜０．０１）。（２）用ＩＬ－１β后ＭＦＲＲ对照组为０．５６４±０．０３２，１０３、１０４、１０５Ｕ／Ｌ组分别为０．４８５±０．０４２、０．４４５±０．０４３、０．４０５±０．０２９（Ｐ＜０．０１）。（３）ＩＬ－６对ＦＲＴＬ－５细胞胞间通讯无明显影响。提示ＴＳＨ可增强ＦＲＴＬ－５细胞间的通讯，ＩＬ－１β可抑制ＦＲＴＬ－５细胞间的通讯，其作用与ＴＳＨ及ＩＬ－１β的剂量有关。

We observed the effects of thyrotropin (TSH), interleukin (IL)1β and IL6 on the intercellular communication of FRTL5 cells with fluorenscence redistribution after photobleaching (FRAP) analysis. FRTL5 cells were cultured and exposed to the different concentrations of TSH, IL1β and IL6 for 12 hours. The mean fluorescence recovery rate (MFRR, %/min) of the cells labelled with carboxyfluocein diacetate (CFDA) after photobleaching was measured with laser scanning cytometry. The MFRR (%/min) of the cells after exposure to TSH was 0.445±0.033 at the control group, 0.679±0.054 at the group of 0.1 U/L, 0.950±0.073 at the group of 1 U/L, and 0.799±0.082 at the group of 5 U/L, respectively (F=11.44,P<0.01). The indicated that TSH could enhance the intercellular communication of FRTL5 cells. The MFRR after exposure to IL1β was 0.564±0.032 at the control group, 0.485±0.042 at the group of 103 U/L, 0.445±0.043 at the group of 104 U/L and 0.405±0.029 at the group of 105 U/L, respectively (F=3.58,P<0.01). The suggested that IL1β could inhibite the intercellular communication of FRTL5 cells. IL6 had no obvious effect on the intercellular communication of FRTL5 cells.