芳香维甲酸乙脂对紫外线诱导的人黑素细胞黑素合成的影响

Effects of Arotinoid Ethylester on UVB induced Melanogenesis of Cultured Human Normal Melanocytes

为了研究芳香维甲酸乙酯（Ｒｏ１３－６２９８）对黑素细胞黑素合成的影响，为应用维甲酸治疗色素沉着性皮肤病提供实验依据。体外培养６例人正常黑素细胞，每日分别经１００ｍＪ／ｃｍ２的中波紫外线（ＵＶＢ）照射，每次照射后立即加入不同浓度（１０－９～１０－５ｍｏｌ／Ｌ）的Ｒｏ１３－６２９８，于第２、４、６天分别用多巴氧化速率法和ＮａＯＨ溶解法测定细胞酪氨酸酶活性和黑素含量。结果：每日用１００ｍＪ／ｃｍ２的ＵＶＢ连续照射培养的黑素细胞，照射４天后细胞酪氨酸酶活性和黑素含量显著高于非照射组（Ｐ＜０．０５～０．０１）；在每次ＵＶＢ照射后加入Ｒｏ１３－６２９８，细胞酪氨酸酶活性和黑素含量则与非照射组无显著性差异；在非照射组未发现Ｒｏ１３－６２９８对细胞黑素合成有明显影响作用。结论：反复小剂量ＵＶＢ照射能通过提高酪氨酸酶活性来实现对培养黑素细胞黑素合成的诱导作用；

The purpose of this study is to invest the effect of arotinoid ethylester (Ro13 6298) on ultraviolet B(UVB) induced melanogenesis of human normal melanocytes (HNM) and provide experimental evidence for treating pigmented diseases of skin. HNM taken from 6 normal persons cultured in vitro were treated with 100mJ/cm 2/day and/or different concentrations (10 -9 ～10 -5 mol/L) of Ro13 6298 respectively, Ro13 6298 were added immediately after each irradiation. 2, 4 and 6 days after the irradiation, tyrosinase activity was estimated by measuring the rate of oxidation of L DOPA, and melanin content by the value of optical density absorbance of NaOH treated HNM. Results showed that tyrosinase activity and melanin content of treated HNM after 4 day UVB irradiation increased significantly (P<0 05～0 01) than those of non irradiated cells, but there was no statistical difference between the non irradiated cell group and Ro13 6298 treatment group. Little effect was found on the melanin synthesis in the non irradiated HNM treated with Ro13 6298 (10 -9 ～10 -5 ). Conclusion: the melanogenesis of cultured HNM can be induced by repeated small dose of UVB irradiations through increasing tyrosinase activity, Ro13 6298 can inhibit melanogenesis induced by UVB irradiation on HNM at lower concentration in vitro.