摘要
目的:为开展骨组织工程研究,建立成骨细胞与支架材料的体外复合培养模型。方法:取新生24h内的Wistar大鼠颅盖骨培养成骨细胞,并将成骨细胞以5×103/孔接种到HA/CS,HA/CS-SF两种支架材料表面常规培养,用倒置显微镜观察细胞形态,通过MTT比色法测出两种支架材料对细胞增殖的影响。结果:培养的成骨细胞呈短梭形或多角形,碱性磷酸酶染色镜下可见胞浆中棕色至黑色颗粒的为阳性细胞。通过MTT chromatometry比色法测定表明HA/CS-SF对细胞增殖有更好的作用。结论:HA/CS-SF具有作为骨组织工程支架材料的可行性。
Objective：To carry out bone tissue engineering research, and to establish a model to provide theoretical bases for the application of scaffold materials in the repair of bone loss. Method：The crania osteoblasts were obtained from newborn Wistar rats within 24 hours. The osteoblasts were transplanted to HA/CS and HA/CS-SF at a density of 5 × 10^3/mL. The biological characteristics of the osteoblasts were observed under the phase-contrast microscope, and the cell proliferation was detected by the assay of MTT. Results ：The cell shape was triangle or polygon, aad black particles could be observed in the endochylema of the cultured ceils with ALP stain. The MTT chromatometry showed that HA/CS--SF had a better effect on the cell proliferation. Conclusion：HA/CS-SF is feasibie as the scaffold materials of bone tissue engineering.
出处
《黑龙江医药科学》
2008年第5期1-2,共2页
Heilongjiang Medicine and Pharmacy
关键词
成骨细胞
细胞培养
支架材料
osteoblast
culture in vitro
scaffold material
