rhIFN-a、rhIL-2与rhTNF-a体外协同抑制SMMC-7721细胞的敏感性实验

In vitro synergistic inhibition of Hela cell by rhIFN-a、rhIL-2 and rhTNF-a

目的:本研究旨在探讨干扰素-a(IFN-a)、白细胞介素-2(IL-2)、肿瘤坏死因子(TNF-a)单一、协同以及与化疗药物DDP的联合作用杀伤肝癌SMMC-7721细胞株的影响。方法:应用四甲基偶氮唑盐比色法(MTT比色法)检测与化疗药物DDP单独或联合应用组及药物作用的12h与24h的光密度值(OD),计算杀伤率。结果:单独应用三种细胞因子时,IFN-a、TNF-a对肝癌SMMC-7721细胞株有细胞毒作用,IL-2对肝癌SMMC-7721细胞株的细胞毒作用不明显。IFN-a、IL-2、TNF-a可以增强DDP的抑瘤作用,与DDP单独作用具有显著性差异(P<0.01)。IFN-a、IL-2、TNF-a联合作用具有协同作用,均与单一应用具有显著性差异(P<0.01)。结论:细胞因子均可以增强顺铂(DDP)的抑瘤作用并与其作用的先后及时间具有依赖关系。IFN-a、TNF-a、IL-2与DDP联合具有协同作用,为肝癌病人化疗提供了理想的参考方案。

Objective：To investigate the effects of rhIFN-a, rhIL-2 and rhTNF-a on the SMMC-7721 cell line in vitro in a single or cooperative form or in combination with chemotherapeutics DDP. Methods： Chemosensitivity of the cells to doxorubicin was analysed by using the MTT assay at 12h and 24h after use of rhIFN-a, rhIL-2 and rhTNF-a alone and in combination with chemotherapeutics DDP. Results ：When the three factors were used alone,IFN-a or TNF-a had toxic action on the liver cancer SMMC-7721 cell line, and when they were used cooperatively, there was significant difference in the effects on the cell line （P 〈 0.01）. All the cytokines had the enhancement of DDP anti-tumor activity in vitro with the dependence of the anti- tumor effect on their action order and time. Conelusion：rhIFN-a,rhIL-2 and rhTNF-a have the synergistic inhibition in combination with DDP,which provides a better chemotherapy choice for patients with liver cancer.