小牛血清培养基用于人群脑膜炎奈瑟菌带菌调查研究

Study on methods for detecting N.meningitidis in healthy people with new bovine serum medium

目的建立一种新型的正常人群脑膜炎奈瑟菌检验方法。方法配制一种小牛血清"双抗"培养基作为脑膜炎奈瑟菌的保菌增菌培养基,用于现场咽拭子采样,带回实验室增菌后再接种平板培养基,分离脑膜炎奈瑟菌,并以常规检测方法作配对比较。结果两次试验新方法检出脑膜炎奈瑟菌14株,常规方法仅在第2次试验时检出脑膜炎奈瑟菌1株。结论应用小牛血清"双抗"培养基建立的新方法适用于A、B、C群脑膜炎奈瑟菌检测,且检测效果明显优于常规的咽拭子直接接种法。

Objective To establish a new method for detecting N. meningitidis in healthy people. Methods A type of new bovine serum and two antibiotics media were produced to keep the life and propagation. With the media, the specimens in the pharynges of healthy people were taken on the spot. The specimens were then brought back to the laboratory, where they were cultured in 37 ~C for 18 to 24 hours. Finally, the apecimens were inoculated on chocolate colour slab medium for detecting N. meningi- tidis. At the same time, N.meningitidis was detected with routine method as the control. Results 14 strains of N. meningitidis were found with the trial method and only 1 strain of N. meningitidis was found in the control group. Conclusions The trial method set up with media of new bovine serum and two antibiotics was suitable for detecting N. meningitidis in healthy people. The positive rate of detecting of the trial method was distinctly higher than that of the routine method of control group.