丙烯腈染毒大鼠不同脑区某些生化指标的变化

Region-specific alterations of some biochemical parameters in brains of subacute acrylonitrile exposed rats

目的探讨亚急性丙烯腈(AN)染毒对大鼠不同脑区某些生化指标的变化。方法32只大鼠随机分成4组,经腹腔注射给予AN0(对照组给予生理氯化钠)、25、50、75 mg/kg体重,每天1次,连续染毒7 d,测定大脑皮层、小脑、纹状体和海马中丙二醛(MDA)和还原型谷胱甘肽(GSH)的含量以及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力。结果与对照组相比,皮层、小脑和海马的MDA含量均呈现剂量依赖性升高,皮层的低剂量组与小脑的高剂量组发生了显著性升高(P<0.05);皮层低剂量组、小脑的中剂量和高剂量组GSH含量较对照组降低有显著性(P<0.05);低剂量组皮层、海马和纹状体SOD活力较对照组降低有显著性(P<0.05);小脑、海马和纹状体在低剂量染毒时GSH-Px活力显著下降(P<0.05)。结论皮层和小脑对MDA增加和GSH含量的减少最为明显,而海马和纹状体对SOD和GSH-Px活力的下降最为明显,提示AN对大鼠氧化性损伤具有脑区特异性。

Objective To examine the effects of acrylonitrile（AN） on some biochemical parameters in various brain regions of adult SD rats. Methods 32 SD rats were randomly divided into 4 groups and given AN at 0（ control）, 25, 50, 75 mg/kg by daily intraperitoneal injection for 7 days, the activities of glutathionepemxidase （ GSH-Px）, supemxide dismutase（SOD） and the levels of glutathione （GSH） and malondialdehyde （MDA） were determined in various brain regions. Results Compared with the control group, the levels of MDA in the cortex, cerebellum and hippocampus were dose-dependently increased, and the contents of GSH were reduced in all brain regions studied. However, the activities of SOD of cortex, hippocampus and striatum in low-dose group were statistically decreased. The activities of GSH-Px of cerebellum, hippocampus and striatum did not show a consistent pattern of response but either increased or decreased in various brain regions. Conclusion Alterations of some biochemical parameters of oxidative stress in rat brains induced by acrylonitrile were region-specific, and changes in variuos regions depended on markers of lipid peroxidation products, GSH contents and antioxidant enzyme activity.