摘要
目的评价牛血清白蛋白∕纳米羟基磷灰石粒子复合物(Bovine Serum Albumin/Nano-Hydroxyapatite,BSA/Nano-HAP)在体外实验中对红细胞的影响。方法溶血实验及渗透脆性实验评价BSA/Nano-HAP对兔红细胞的影响,并对材料与红细胞共培养后做细胞形态学观察,Bradford法检测不同粒径的HAP与BSA的吸附量,绘制吸附等温线,Nano-HAP与BSA共吸附后作红外光谱分析。结果BSA/Nano-HAP与兔红细胞共培养后未引起溶血和细胞聚集现象,Nano-HAP对BSA有强烈的吸附作用,作用位点主要在BSA的酰胺I带、酰胺II带、[COO-]基团与羟基磷灰石的[Ca2+]、[OH-]、[PO43-]基团上。结论BSA/Nano-HAP与红细胞共培养后,细胞形态正常,并且保持了红细胞正常的悬浮性,有望成为一种血液相容性较为理想的生物材料。
Objective Evaluating Bovine Serum Albumin/Nano-Hydroxyapatite （BSA/Nano-HAP） effect on erythrocyte in vitro. Method Evaluating the effect BSA/Nano-HAP on rabbit erythrocyte with haemolysis experiment and erythrocyte osmotic fragility test and observing cell morphology on co-culture of both. Determining amount of BSA ad- sorbed on different size hydroxyapatite nanoparticles by Bradford and drawing adsorption isotherm and analyzing interaction by infrared spectrum. Result Haemolysis and erythrocyte aggregation can＇t be found after Nano-HAP and BSA co-cultured. Moreover, a striking adsorbability appeared between hydroxyapatite nanoparticles and BSA, the site maybe amide Ⅰ band, amide Ⅱ band and [COO] group of BSA binding to [Ca^2＋],[OH^-] and [PO4^3-] group of Nano-HAP. Conclusion BSA/Nano-HAP will be expected a ideal haemo-compatibility materials since it maintains erythroeyte＇s normal morphous and floaty nature after co-cultured.
出处
《生物骨科材料与临床研究》
CAS
2008年第5期4-7,11,共5页
Orthopaedic Biomechanics Materials and Clinical Study
