高效液相色谱法测定乙肝抗毒颗粒中芍药苷的含量

Determination of Paeoniflorin in Yigan Kangdu Granules by HPLC

目的建立测定乙肝抗毒颗粒中芍药苷含量的方法。方法采用高效液相色谱法,以Agilent Eclipse XDB-C18为分析柱(4.6mm×250mm,5μm),流动相为乙腈-水(20∶80),检测波长230nm。结果芍药苷在0.04～0.48μg范围内线性关系良好(r=0.9994),平均回收率为98.52%,RSD为1.50%(n=6)。结论本方法简便、快速、分离度好,可用于乙肝抗毒颗粒的质量控制。

Objective To establish a method for the determination of paeoniflorin in Yigan Kangdu Granules. Methods The analysis was carried out on an Eclipse XDB-C18 column with a mobile phase of acetonitrile-water （20 ： 80）. The detection wavelength was 230 nm. Results The linearity was obtained over the range of 0.04-0.48 μg （r =0.9994） for paeoniflorin. The average recovery was 98.52%, RSD was 1.50% （n=6）. Conclusion The method is simple, accurate, and suitable for the quality control of Yigan Kangdu Granules.