胶束薄层层析法分离苦参散

Separation of kurainone powder medicine using micellar thin layer chromatography

目的：研究苦参散主要成份的分离鉴定，为该药建立企业质量标准提供实验依据。方法：胶束薄层层析。结果：ＣＴＡＢ、ＳＤＳ胶束水溶液浓度分别为０．００６ｍｏｌ·Ｌ￣（－１０）．０４ｍｏｌ·Ｌ￣（－１）时，甘草的Ｒｆ值为０．５０，０．５２；黄芩的Ｒｆ值为０．７２，０．６９；苦参的Ｒｆ值为０．３０，０．３７。分离效果最好。结论：胶束薄层层析法，可做为苦参散中各主要成份含量的分离方法。

Objective:To study main composition of kurainone powder medicine which is separated Methods:Using micellarthin Layer Chromatography ReSults: Surface active agent CTAB concentration is 0.006mol. L^(-1), SDS concentration is 0.04mol. L^(-1),Licorice root Rf CTAB= 0.50 Rf SDS=0.52, Baicalein Rf CTAB=0.72 Rf SDS=0.69,kurainoe Rf CTAB=0.30, Rf SDS=0.37 It's separate ettection was batter than other method .Conclusions: Micellar thin layer chromatography may be to ma-jor method which kurainone Powder medicine's main conpeition. Such condtision Provied a strong experimented evidencefor kurainone Powder medicine on quality standard of enterprise.