慢性胆囊炎胆囊粘膜T淋巴细胞亚群分布及意义的实验研究

Distribution and significance of T lymphocyte subsets of gallbladder mucosas in chronic calculius cholecystitis

目的：为研究胆囊炎症时胆囊局部，细胞免疫功能，探讨免疫反应与炎症发展和病理改变的关系。方法：用ＯＫ系列单克隆抗体及ＡＢＣ染色法对８例正常人和２７例慢性结石性胆囊炎病人胆囊粘膜Ｔ淋巴细胞亚群进行了检测。结果：慢性结石性胆囊炎病人胆囊粘膜固有层内有大量淋巴细胞浸润，Ｔ３细胞、Ｔ４细胞和Ｔ８细胞均明显增高（Ｐ＜０．００１），Ｔ４／Ｔ８比值为１．７５±０．１明显高于正常１．１８±０．３４。而且随病程延长和症状加重，胆囊固有层Ｔ淋巴细胞亚群数量明显增高。结论：慢性结石性胆囊炎时胆囊粘膜Ｔ淋巴细胞亚群间比例失调及异常分布与胆囊组织损伤和纤维化有关。

Objective: In order to study local Cell-Mediated immunological function of the gall-bladder mucosas as well asrelationship between humen immuno-reaction and inflammation gow and pathologic changs of the gall-bladder wall of the pa-tients.Methods:T Lymphoceyte subsets of the gall-bladders mucosas in 8 health men and 27 patients of chronic calculouscholecystitis were determined by utilizing McAbs and ABC methed. Resuts: A large number of T lymphocytes infiltrate inIamina propria of the patients gall-bladder wall, a number T3+ Cell, T4+ cell and T8+ cell all abriously increase in contrast toincontrol (P less then 0.001). The retio of T4+ cell to T8+ cell in the patients(1.75 ± 0. 1) was significanily increased ascompared with that in control(1. 18± 0.34) (P less then 0.01), but a number of T cell subsets was clearly enhanced alongwith last of clinic diseases course and symptoms worsened of the patients.Conclusions:The tissue damage and fibrosis ofgall-bladder wall in chionic calculous cholecystitis can relate to lase adjust of propation between T lympheeyte subsets andabnormal distribution.