摘要
从采自大连近海的红藻鸭毛藻(Symphyocladia latiuscula)中分离到一株肉座菌目真菌(Hypocreales sp.),对其发酵代谢产物的化学成分进行了研究。利用正相硅胶柱层析、葡聚糖凝胶SephadexLH-20柱层析、制备薄层层析(PTLC)以及重结晶等分离手段,并通过一维、二维核磁共振技术、质谱技术等从该菌发酵液中分离鉴定了10个化合物:双酚A(1);邻羟基苯甲酸(2);吲哚甲酸(3);吲哚乙酸(4);N-乙酰色胺(5);(22E,24R)-麦角甾-7,9,22-三烯-3β-醇(6);过氧化麦角甾醇(7);(22E,24R)-5α,6α-环氧麦角甾-8,22-二烯-3β,7α-二醇(8);(22E,24R)-麦角甾-7,22-二烯-6β-甲氧基-3β,5α-二醇(9);啤酒甾醇(10)。这些化合物均为首次从该菌中分离得到,其中化合物1为首次作为天然产物分离得到。
From the fermentation broth of Hypocreales sp. , an endophytic fungus derived from the red alga Symphyocladia latiuscula, ten compounds were isolated by the normal phase silica gel, Sephadex LH-20 column chromatography separation, and by the preparative thin layer chromatography (PTLC) as well as by re-crystallization. Structures of purified compounds were elucidated by analysis of spectroscopic data including MS, 1D and 2D NMR as bisphenol A (1), salicylic acid (2), 3-indoleformic acid (3), 3-indoleacetic acid (4), N-aeetyltryptamine (5), 22E, 24R-ergosta-7,9,22-trien-3β-ol (6), ergosterol peroxide (7), and 22E, 24R-5α, 6α-epoxyergosta-8, 22-dien-3β, 7α-diol (8), ergosta-7, 22-dien-6β-methoxy-3β, 5α-diol (9), cerevisterol (10). All the compounds were obtained from this fungus for the first time and compound (1) was reported as a new natural product.
出处
《海洋科学》
CAS
CSCD
北大核心
2008年第11期51-55,共5页
Marine Sciences
基金
国家自然科学基金项目(30530080)
山东省科技发展计划项目(2006GG2205023)
海洋药物教育部重点实验室开放基金项目[KLMD(OUC)2004]