鸭毛藻内生真菌Hypocreales sp．的化学成分研究

Chemical constituents of Hypocreales sp.,an endophytic fungus derived from the red alga Symphyocladia latiuscula

从采自大连近海的红藻鸭毛藻(Symphyocladia latiuscula)中分离到一株肉座菌目真菌(Hypocreales sp.),对其发酵代谢产物的化学成分进行了研究。利用正相硅胶柱层析、葡聚糖凝胶SephadexLH-20柱层析、制备薄层层析(PTLC)以及重结晶等分离手段,并通过一维、二维核磁共振技术、质谱技术等从该菌发酵液中分离鉴定了10个化合物:双酚A(1);邻羟基苯甲酸(2);吲哚甲酸(3);吲哚乙酸(4);N-乙酰色胺(5);(22E,24R)-麦角甾-7,9,22-三烯-3β-醇(6);过氧化麦角甾醇(7);(22E,24R)-5α,6α-环氧麦角甾-8,22-二烯-3β,7α-二醇(8);(22E,24R)-麦角甾-7,22-二烯-6β-甲氧基-3β,5α-二醇(9);啤酒甾醇(10)。这些化合物均为首次从该菌中分离得到,其中化合物1为首次作为天然产物分离得到。

From the fermentation broth of Hypocreales sp. , an endophytic fungus derived from the red alga Symphyocladia latiuscula, ten compounds were isolated by the normal phase silica gel, Sephadex LH-20 column chromatography separation, and by the preparative thin layer chromatography （PTLC） as well as by re-crystallization. Structures of purified compounds were elucidated by analysis of spectroscopic data including MS, 1D and 2D NMR as bisphenol A （1）, salicylic acid （2）, 3-indoleformic acid （3）, 3-indoleacetic acid （4）, N-aeetyltryptamine （5）, 22E, 24R-ergosta-7,9,22-trien-3β-ol （6）, ergosterol peroxide （7）, and 22E, 24R-5α, 6α-epoxyergosta-8, 22-dien-3β, 7α-diol （8）, ergosta-7, 22-dien-6β-methoxy-3β, 5α-diol （9）, cerevisterol （10）. All the compounds were obtained from this fungus for the first time and compound （1） was reported as a new natural product.