陡脉冲致肿瘤不可逆性电击穿的场强阈值研究

Electric Intensity Threshold of Irreversible Electric Breakdown of Rabbit Liver VX_2 Tumor Under Steep Pulse Electric Field

为了明确陡脉冲电场致在体肿瘤细胞不可逆性电击穿的电场强度阈值,以新西兰大白兔肝脏VX2肿瘤组织为研究对象,建立了陡脉冲在均匀介质中的有限元模型;采用电场仿真和荷瘤动物实验相结合的方法,用不同电压峰值的陡脉冲电场分别处理荷瘤组织,并用经病理切片描绘得出的电场杀伤覆盖范围同理论仿真模型相互验证,以求得陡脉冲电场在肿瘤组织中的分布,进而求出其电场强度阈值。结果表明:理论仿真与动物实验相互吻合;根据肿瘤组织的坏死轮廓和仿真结果初步判定陡脉冲电场致在体兔肝VX2肿瘤组织不可逆性电击穿的电场强度阈值为838±46 V/cm。本研究为治疗参数的恰当选择提供参考,只要电场覆盖范围内肿瘤区域的最小电场强度略大于肿瘤细胞不可逆性电击穿阈值,即可使肿瘤组织得到有效杀伤而正常组织受到最小损伤。

To study the VX2 liver tumor of white rabbit, the finite element model of steep pulse in the homogeneous medium was established. The method of the combination of animal experiment and electric field simulation was adopted to treat the tumor with steep pulse electric field of the different peak values of voltage, and the electric field＇s covering area between the description of pathological section and theoretical simulation-model was compared to ob rain the distribution of the steep pulse electric field in the tumor. It is confirmed the electric intensity threshold value leads to the irreversible electrical breakdown. Results show that the theoretical simulation and the animal experiment are anastomotic. According to the necrotic contour of tumor and the simulation results, the electric intensity threshold value of steep pulse leading to the irreversible electrical breakdown is 838 46V/cm. This offers a reference to choose suitable treatment parameters. As long as the minimum electric intensity in the tumor area is higher than the threshold value which tumor cell leads to the irreversible electrical breakdown, it could kill the tumor tissue effectively and damage the normal tissue lightly.