白藜芦醇诱导KG-1细胞凋亡作用机制的初步研究

Preliminary Study on the Mechanism of Apoptosis Induced by Resveratrol in KG-1 Cells

通过观察白藜芦醇诱导人急性白血病细胞株KG-1凋亡的作用,检测bcl-2、caspase-3的表达,探讨白藜芦醇诱导白血病细胞凋亡的作用机制。采用噻唑蓝(MTT)比色法分析细胞生长状态;瑞-吉染色、透射电镜观察KG-1细胞凋亡的形态学变化;流式细胞术(FCM)测定细胞凋亡与周期分布;半定量RT-PCR检测bcl-2 mRNA、caspase-3 mRNA表达水平。结果显示白藜芦醇可明显抑制KG-1细胞增殖(P<0.01),与对照组相比,实验组使细胞发生S期阻滞(P<0.01),促进细胞凋亡(P<0.01),使bcl-2的表达下调,上调caspase-3的表达(P<0.05)。结论:白藜芦醇能诱导KG-1细胞凋亡,其机制可能与下调bcl-2、上调caspase-3表达水平有关。

In order to explore the possible mechanism of apoptosis effect induced by resveratrol in human acute leukemia KG-1 cells, the suppressive effect of resveratrol on proliferation of KG-1 cells was analyzed with MTT method. Wring-Giemsa Staining, transmission electron microscope technique were used to detect the apoptosis status of KG-1 cells. The cell cycle and apoptosis percentage of KG-1 ceils treated with resveratrol were detected by flow cytometry. The expression of bcl-2 and caspase-3 mRNA was assessed by semi-quantitative RT-PCR. The results showed that resveratrol can obviously inhibit proliferation of KG-1 cells （P 〈0.01 ）. Compared to control group, the cell number in S phase of KG- 1 cells treated with resveratrol was increased（ P 〈0.01 ） , and the apoptosis rate increased significantly （P 〈 0.01 ） and the expression of bcl-2 reduced, caspase-3 increased （ P 〈 0.05 ）. Resveratrol has significant inhibitory effect in KG-1 cells in vitro, it is probably related to restrain the expression of bcl- 2 and improve the expression of caspase-3.