脑梗死患者血管紧张素原T704C基因多态性和血管紧张素Ⅱ受体-1 A1166C的基因多态性分析

Relationship Between the AGT Gene and AT1R Gene Polymorphisms and Cerebral Infarction

目的:研究血管紧张素原(AGT)T704C基因多态性和血管紧张素Ⅱ受体-1(AT1R)A1166C与脑梗死(CI)的关系。方法:应用聚合酶链式反应—限制性片断长度多态性(PCR-RFLP)技术检测77名CI患者和98名健康人群的AGT和AT1R基因型和等位基因频率并进行比较和分析。并运用Logistic回归分析遗传因素对CI的作用。结果:CI组AGT CC基因型频率(63.6%)及C等位基因频率(79.9%)显著高于对照组(34.7%,61.2%),P<0.05;CI组的AT1R AC基因型频率(61.1%)及C等位基因频率(30.5%)与对照组(8.2%,4.1%)比较差异有统计学意义(P<0.05)。Logistic回归分析后,AGT C等位基因参与增加CI的发生概率,发病的相对危险度(OR)为2.513,P<0.05。AT1R C等位基因参与增加CI的发生概率,发病的相对危险度(OR)为10.322,P<0.05。在同时携带AGT C等位基因和AT1R C等位基因的个体患CI的危险度为14.325,P<0.05。结论:AGT基因多态性可能是CI发病的遗传因素;与AT1R C等位基因间具有协同致CI作用。

Objective： To identify an interaction between angiotensinogen（AGT） gene polymorphisms and angiotensin Ⅱ type Ⅰ receptor （AT1R） gene polymorphisms on the risk of cerebral infarction. Methods： AGT genotypes and AT1R genotypes were investigated with the methods of PCR - RLFP in 77 patients with cerebral infarction （CI） and 98 healthy individuals controls. Fatalness of genetic factors toward CI were analyzed by Logistic regression. Results： AGT T704C gene polymorphism revealed significant difference of CC genotype and C allelic distribution in CI patients （63.6 %, 79.9 % ） and controls（34.7 %, 61.2 % ）. There were significantly difference in the frequencies of AT1R Al166C genotypes and C allele among CI patients（61.1%, 30.5 % ） and controls（8.2 %, 4.1% ）;Logistic regression analysis showed the polymorphsim of AGT gene was therisk factor for cerebral infarction （OR = 2. 513, P 〈 0.05） ;the polymorphsim of AT1R gene was the risk factor for cerebral infarction （OR = 10. 322, P 〈 0.05）. The polymorphsim of AGT gene and AT1R allele might have coordinating function in the pathogenesis of cerebral infarction （OR = 14. 325, P 〈 0.05）. Conclusion： The polymorphism of AGT is related to the incidence of CI; There were synergistic effects of AGT and AT1R gene polymorphisms on the risk of CI.