西南横断山区野生狗牙根遗传多样性的RAMP分析

Genetic Diversity of Cynodon dactylon accessions Based on RAMP Markers in Hengduan Mountains from Southwest of China

采用RAMP标记技术,分析了西南横断山区25份野生狗牙根材料的遗传多样性。结果表明,被测材料间RAMP标记多态性较高。10个引物组合扩增出的56条带中,39条具多态性,PPB为69.64%。每个引物组合可扩增出2~6条多态性带,平均3.9条。RAMP标记遗传相似性系数（GS）变化范围为0.6667~0.9474,平均值为0.8129。RAMP标记可将所有25份狗牙根材料区分开,供试材料可以明显的划分为4大类。聚类结果与材料的地理分布有一定关系。据此认为,西南横断山区野生狗牙根种质资源在分子水平确实存在较大的遗传差异,RAMP是评价狗牙根遗传多样性的有效方法。

Genetic diversity of 25 Cynodon dactylon accessions from Hengduan Mountains from southwest of China were analyzed by using RAMP （random amplified microsatellite polymorphism） markers. The results indicated that the polymorphism among the tested accessions was plentiful. A total of 56 bands were amplified based on 10 primer combinations, of which 39 bands were polymorphic, the percentage of polymorphic bands was 69.64. Two to six polymorphic bands could be amphfied from each primer combination,with an average of 3.9 bands. The RAMP-based genetic similarity （GS） among 25 bermudagrass accessions ranged from 0. 666 7 to 0.947 4 ,with a mean of 0.812 9. All the accessions could be distinguished by RAMP markers. The cluster analysis resluts showed that 25 accessions were classfied into 4 groups, which were correlated with the geo- graphical distribution of the accessions to some degree. These results suggested that there actually existed plentiful genetic diversity of C. dactylon. RAMP marker was a useful method for analyzing the genetic diversity among C. dactylon accessions.