毛细管电泳法测定盐酸洛美沙星片的含量

Determination of Lomefloxacine Hydrochloride Tablets by High Performance Capillary Electrophoresis

目的建立毛细管电泳法（HPCE）测定盐酸洛美沙星片的含量。方法以0．2mol／L NaH2PO4-H3PO4缓冲液（pH4．0）为背景电解质，50cm×75μm未涂层空心毛细管柱为分离通道，检测波长为282nm，运行电压25kV，压力进样（0．5psi×3s），柱温25℃，采用峰面积外标法定量。结果在选定的电泳条件下，洛美沙星的浓度在50．00～500．00mg／L范围内与峰面积呈良好的线性关系（r=0．9995）；低、中、高三种浓度（n=3）的平均回收率分别为99．94％、99．94％与96．25％；方法的检测限（S／N=3）为5．00mg／L；日内和日间峰迁移时间的RSD分别2．17％、1．13％，日内和日间峰面积的RSD分别为3．94％、1．97％。结论本法试剂用量少、简便、快速、准确，可用于洛美沙星的质量检控。

Objective To establish an easy method for Lomefloxacine hydrochloride tablets by high performance capillary electrophoresis（HPCE）. Methods The elcctrophoresis buffer consis- ted of 0.2mol/L NaH2PO4 -H3PO4 （pH4.0）;The analysis was performed in an uncoated capillary of 50cm×751μm; The UV detection wave length was 282nm ;The running votage was 25kV ;The injection pressure and time was 0. 5psi and 3s;The column temperature was 25℃. External calibration curve of peak area was used. Results There was excellent linearity between the peak area and concentration of lomefloxacine in the range of 50. 00 -500. 00mg/L. The correlation coefficient was 0. 9995. The average recovery were 99.94% ,99.94% ,96.25% respectively at the lower, middle and higher concentration （ n = 3 ）. The relative standard deviation （RSD） value for migration time of intra - assay and inter -assay were 2. 17% and 1.13% respectively. The relative standard deviation （RSD）value for peak area of intra - assay and inter-assay were 3.94% and 1.97% respectively. Conclusion The method is less solvent consumption, simple, rapid, accurate and is beneficial for quality analysis and control of lomefloxacine.