桑叶中1-脱氧野尻霉素的纯化及含量测定

Purification and Determination of DNJ from Leaves of Morus alba

目的提取纯化桑叶中1-脱氧野尻霉素(DNJ),并用柱前衍生化高效液相法进行含量测定。方法桑叶加0.05mol·L-1盐酸溶液超声提取,提取物进行反相柱色谱纯化,纯化物用柱前衍生化-高效液相-荧光检测法测定DNJ的含量。结果桑叶中DNJ的含量为0.27%～0.49%,纯化物中DNJ的含量达到了10.7%,DNJ在0.6～60μg·mL-1内浓度与峰面积呈良好的线性关系(r=0.9997),平均回收率为104.46%。结论运用反相色谱技术分离纯化DNJ简单易行,含量测定方法精确,重复性好。

OBJECTIVE To extract and purify 1-deoxynojirimycin（DNJ） from leaves of Morus alba L. and establish a precolumn derivatization HPLC method to determine the content of DNJ. METHODS DNJ in Morus alba L. leaves were extracted crudely with 0.05 mol·L^-1 HCl by uhrasonie, the crude extracts were isolated and purified with reversed-phase column chromatography, then the purified products were determined by HPLC. RESULTS Three leave materials collected from different areas were determined and the contents of DNJ in leave were fluctuated from 0.27% to 0.49%. The DNJ content in purified products was 10.7%. The calibration curve was linear over the range of 0.6 - 60 g·mL^-1 （ r = 0. 999 7） and the average recovery was 104.46%. CONCLUSION A simple technology for the extraction and purification of DNJ in Morus alba L. Leaves is investigated. The determination method for DNJ is accurate and repeatable.