不同产地白花蛇舌草中对香豆酸含量测定的快速方法

A Rapid Method for the Quantitative Determination of P-coumaric Acid in Hedyotis diffusa from Different Sources

目的建立实用的不同产地白花蛇舌草中对香豆酸的高效液相色谱含量测定方法。方法采用AgilentEclipse×DB-C18(250mm×4.6mm,5μm)色谱柱,乙腈-0.1%磷酸水溶液梯度洗脱,流速1.0mL·min-1,检测波长230nm,柱温30℃,进样量20μL,测定了6批不同来源的白花蛇舌草药材。结果线性范围4～64μg·mL-1,r=0.9998,平均回收率(n=6)为96.86%,RSD=0.22%。结论本法简便快速,重现性良好,可作为白花蛇舌草药材质量控制的有效方法。

OBJECTIVE To establish a practical HPLC method for the determination of P-coumaric acid in Hedyotis diffusa Willd. METHODS The chromatographic conditions included Agilent Eclipse DB-C18 （250 mm×4.6 mm,5 μm） column, mobile phase of acetonitrile-0.1% phosphate solution, column temperature of 30 ℃ , flow rate of 1.0 mL·min^-1 , wavelength of 230 nm, and inject volume of 20 μL. RESULTS The linear range was 4-64 μg·mL^-1（r=0.999 8）, the average recovery （n =6） was 96.86%, and the relative standard deviation （RSD） was 0.22%. CONCLUSION The method is rapid and simple with good precision and reproducibility. It can he used for quality control of Hedyotis diffusa Willd.