摘要
目的建立高效液相色谱法测定参术儿康糖浆中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量。方法采用TechsphereC18色谱柱;流动相为乙腈-水(16∶84);检测波长为320nm;柱温为40℃。结果2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷进样量在0.04496~0.22480μg内与峰面积积分值呈良好的线性关系(r=0.9999);平均加样回收率为100.25%(RSD=0.69%,n=5)。结论方法简便、准确,可作为该制剂的质量控制方法。
OBJECTIVE To establish an HPLC method for the determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D- glucoside in Shenzhu Erkang syrup. METHODS The Techsphere C18 column was used as the stationary phase with the mobile phase of acetonitrile-water( 16: 84). The detection wavelength was 320 nm and the column temperature was 40 ℃. RESULTS The linear range of 2,3,5,4 ' -tetrahydroxystilbene-2-O-β-D-glucoside was 0. 044 96 -0. 224 80 μg( r = 0. 999 9). The average recovery was 100.25% with the RSD of 0.69% (n = 5). CONCLUSION The method is convenient and accurate. It ean be used for the quality control of 2,3,5,4' -tetrahydroxystilbene-2-O-β-D-glueoside in Shenzhu Erkang syrup.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2008年第5期436-438,共3页
Chinese Journal of Modern Applied Pharmacy