PCR与ELISA在检测乙型肝炎病毒标志物中的应用比较

APPLIED COMPARISION IN DETECTINC THE MARKERS OF HBV BY MEANS OF PCR AND ELISA

目的：观察PCR与ELISA法在检测乙肝病毒标志物中的差异。方法：用PCR和ELISA法对803例疑似乙肝者做了HBVDNA和乙肝五项指标检查，并进行比较。结果：在HBsAg（＋）的736例中HBVDNA阳性率58．77％。HBeAg（＋）的353例中HBDNA阳性率为96．3％。HBeAg（－）的408例中HBVDNA阳性率为23．0％。HBsAg和HBeAg均（－）的25例中HBVDNA阳性率为8．0％。结论：不能单靠HBeAg阳性判断HBV复制，对HBeAg（－）者有必要再进行PCR检测HBVDNA。

Objective To obsevve the mathod of PCR and ELISA in detecting the differentes of the markers of the HBV(hepatitis B virus). Methods 803 cases of suspected HBV carriers were examined in their HBVDNA and 5 indexes of HBV by means of PCR and ELISA and the comparision was made. Results in 796 cases of HBsAg(+),the positive rate of HBVDNA was 58. 7%, in 353 cases of HBeAg(+),the positive rate was 96. 3%,in 408 cases HBeAg(-), the positive rate was 8.0%. Conclusions Only by depending on the positive rate of HBeAg can the replication of HBV not be determined and it is necessary that the carriers of HBeAg be determined again in their HBVDNA by means of PCR.