HMC毒素培养滤液诱导C103玉米抗小斑病的研究(英文)

Induced Resistance to Bipolaris maydis Disease of Maize C103 by Culture Filtrate of HMC Toxin

以1对同核异质玉米C103自交系为试材,采用离体叶片法检测适宜浓度的玉米小斑病菌C小种(HMC)毒素培养滤液来诱导玉米的抗病性。结果表明:对照的玉米病斑面积是处理组的7.9～12.2倍,差异极显著,并以1∶60预处理效果最佳;经0～72 h的动态检测,与对照相比,诱导玉米的过氧化物酶活性平均提高了57.8%,苯丙氨酸解氨酶活性平均提高了52.9%,有害物质丙二醛的含量平均降低了38.2%;低浓度HMC毒素培养滤液能够作为激发子诱导专化寄主玉米获得抗病性。

In the experiment induced disease-resistance of maize by culture filtrate of HMC toxin was investigated. A pair of homokaryon maize C103 inbred lines were used as the test material. The pathogenicity was measured by the detached leaf method in order to find moderately diluted concentration for inducing disease-resistance. The results showed that lesion area decreased by 7. 9- 12. 2 times. It was significantly different by ANOVA. Among the treatments, the effect of 1 ： 60 is better than 1 ： 50;POD and PAL activities increased by 57.8% and 52. 9 % respectively. However, MDA content decreased from by 38. 2 % compared with CK during 0-72 h inspection. In short,moderately diluted（1 ： 60） culture filtrate of HMC toxin could function as an elicitor to induce host disease-resistance in maize.