光皮木瓜齐墩果酸超声波辅助提取及纯化工艺

Ultrasonic wave-assisted extraction and purification of oleanolic acid from Chaenomeles sinensis (Thouin) Koehne

以光皮木瓜为原料,利用超声波辅助提取及大孔吸附树脂纯化齐墩果酸。通过二次正交旋转组合设计优化提取工艺条件,采用静态吸附和解吸试验筛选合适的大孔树脂,并运用动态吸附和解吸试验验优化纯化条件。结果表明,超声波辅助提取最佳工艺条件为:超声功率600 W、提取温度53℃、液料比8:1、超声波提取70 min,回流提取120 min,齐墩果酸得率为3.000 mg/g;XDA-1型大孔树脂对齐墩果酸具有较好的吸附和解吸效果,最佳纯化工艺条件为上柱液浓度0.794 mg/mL,吸附流速0.5 mL/min;以90%浓度乙醇洗脱,洗脱流速1.0 mL/min,纯化后得提取物中齐墩果酸纯度可达26.55%。

The ultrasonic wave-assisted extraction technology and macroporous resin was used to extract and purify oleanolic acid from dried fruit of Chaenomeles sinensis（Thouin） Koehne through quadratic rotation-orthogonal combination design. Macroporous resin was chosen with static adsorption and desorption experiments, and the optimum purification parameters were established with dynamic adsorption and desorption experiments. Results indicate that the optimum extraction conditions are as follows： ultrasonic power is 600W, temperature is 53℃, the ratio of liquid to solid is 8 ： 1, ultrasonic treating time is 70min, reflux extraction time is 120min, the extraction rate of oleanolic acid is 3.000 mg/g; XDA-1 resin is favorable in purification of OA in Chaenomeles sinensis（Thouin） Koehne, the optimum purification conditions are found to be 0.794 mg/mL of OA concentration, the flow rate of adsorption is 0.5 flow rate of elutant is 1.0 mL/min, the content of OA can be increased mL/min; the desorption solvent is 90% ethanol, and the up to 26.55% after being purified by XDA-1 resin.