利湿化痰健脾法对高脂血症大鼠一氧化氮合酶、血管内皮素基因表达的影响

Influence of Removing Dampness,Melting Phlegm and Tonifying Spleen Method on Nitric Oxide Synthase and Gene Expression of Vascular Endothelial in Hyperlipidemia Rats

目的观察利湿化痰健脾法对高脂血症大鼠血脂的影响及对血管内皮的保护作用。方法动物随机分成6组,高脂饲料喂养15d,于第16日眼眶取血查血脂,待证实造模成功后分别按不同实验要求灌胃给药。第37日检测一氧化氮合酶及血管内皮素基因表达。结果用药后各组实验大鼠一氧化氮合酶表达与正常组比较,P<0.01;治疗组与模型组比较,P<0.01。用药后各组大鼠血管内皮素基因RT-PCR产物光密度值与正常组比较,P<0.01;治疗组与模型组比较,P<0.01。结论利湿化痰健脾法通过升高高脂血症大鼠动脉壁内一氧化氮合酶的活性、降低血管内皮素基因的表达而达到调节实验大鼠的血脂水平、保护血管内皮的作用。

Objective To observe the law of removing dampness, melting phlegm and tonifying spleen on blood lipids in hyperlipidemia rat and its vascular endothelial protective effect. Methods Animals were randomly divided into 6 groups, the high-fat diet of 35 d, 16 d orbital check blood lipids to confirm modeling success and gavage was given by different experimental requirements. NOS and vascular endothelial gene expression were determined at 3rd and 7th day. Results After treatment, NOS expression in experimental rats compared with the normal group, P〈0.01, and the treatment group compared with the model group, P〈0.01. Vascular ET-1 mRNA RT-PCR product optical density value of experimental group compared with the normal group, P〈0.01, and the treatment group compared with the model group, P〈0.01. Conclusion The law of removing dampness, melting phlegm and tonifying spleen can increase arterial wall NOS activity and reduce vascular endothelial gene expression in hyperlipidemia rats, so as to regulate the level of blood lipids and protect vascular endothelium.