尿畅舒胶囊对去势雌鼠血清NO、GnRH及膀胱cGMP含量的影响

Effect of Niaochangshu Capsule on Serum NO,GnRH and Bladder cGMP of Emasculated Female Rats

目的通过观察尿畅舒胶囊对去势雌鼠血清一氧化氮(NO)、促性腺激素释放激素(GnRH)及膀胱组织中环磷酸鸟苷(cGMP)含量的影响,探讨尿畅舒胶囊治疗绝经后尿道综合征的作用机理。方法成年健康SD雌性大鼠48只,随机分为空白组、模型组、尼尔雌醇对照组及尿畅舒胶囊治疗组。去卵巢建立大鼠雌激素缺乏模型。灌胃给药4周后,取血清测定NO、GnRH的含量,取膀胱组织测定cGMP含量。结果空白组、对照组及治疗组大鼠血清NO浓度及膀胱组织cGMP含量均明显高于模型组,差异有统计学意义(P<0.05);治疗组与对照组比较,差异有统计学意义(P<0.05)。4组大鼠血清GnRH的浓度差异无统计学意义(P>0.05)。结论尿畅舒胶囊能有效提高去势雌鼠血清NO的浓度及膀胱组织中cGMP含量,效果优于尼尔雌醇。

Objective The change of nitrogen oxide （NO） and gonadotropic releasing hormone （GnRH） in serum and cyclic guanosine monophosphate （eGMP） in bladder of emasculated female rat after treatment by Niaochangshu capsule was studied to investigate the mechanism of action of Niaochangshu capsule. Methods A total of 48 adult Sprague-Dawley female rats were randomly divided into normal group, model group, control group with Nilestriol and trial group with Niaochangshu capsule. Resect the ovaries to make the estrogen-lack model. After 4 weeks intragastric administration, the serum NO and GnRH and bladder cGMP of all rats were examined. Results The content of serum NO and bladder cGMP were significant lower in the model group than the other three groups （P〈0.05） and different between the trial group and the control group （P〈0.05）. There was no difference in the four groups of the content of serum GnRH （P〉0.05）. Conclusions In emasculated female rats, the content of serum NO and bladder cGMP can be significantly increased by the Niaochangshu capsule, and it is better than Nilestrio.