白花蛇舌草诱导人肺巨细胞癌细胞株PG细胞凋亡的初步研究

Preliminary Study on Apotosis of PG Cells Induced by Herba Hedyotis Diffusae

目的研究白花蛇舌草诱导人肺巨细胞癌细胞株PG细胞凋亡的作用,以探讨其抗肿瘤的作用机制。方法用不同浓度的白花蛇舌草含药血清分别处理PG细胞,采用流式细胞仪分析白花蛇舌草对PG细胞在细胞周期各个时相不同DNA含量和细胞凋亡峰的影响。结果经白花蛇舌草含药血清作用的PG细胞在非凋亡细胞二倍体峰(G1)之前出现典型的凋亡细胞峰,不同浓度的含药血清对PG细胞的作用存在明显的量效关系,凋亡率分别为6.86%、9.77%、11.69%。结论诱导肿瘤细胞凋亡可能是白花蛇舌草抗肿瘤作用机理之一。

Objective To investigate the anticancer mechanism of Herba Hedyotis Diffusae on PG cell by observing the action of Herba Hedyotis Diffusae in inducing apoptosis of PG cells. Method After treating PG cells with different doses of drug containing serum of Herba Hedyotis Diffusae, FCM was used to detect the apoptosis of PG cell. Result After treating PG cell with Herba Hedyotis Diffusae, typical apoptosis characteristics could be seen and apoptosis rates were increased with the relation of dosage-effect, which were 6.86% , 9.77% and 11.69%, respectively. Conclusion Inducing the apoptosis of tumor cells maybe part of the anti-tumor mechanism of Herba Hedyotis Diffusae.