HPLC法测定甲肿消及大鼠血清中土贝母苷甲的含量

Determination of Tubeimoside Ⅰ in Jiazhongxiao Preparation and Serum of Rat

目的建立甲肿消制剂及灌胃给予甲肿消后大鼠血清中土贝母苷甲的含量测定方法。方法采用RP-HPLC法,甲肿消制剂用甲醇萃取,血清样品采用C18SPE固相萃取柱(3mL,200mg,60μm)处理,甲醇洗脱;色谱柱:DiamonsilC18(250mm×4.6mm,5μm)和Easyguard保护柱;流动相:甲醇-水(65∶35),检测波长:214nm,流速:1mL/min。结果甲肿消制剂及大鼠血清中土贝母苷甲的回收率分别为(103.49±3.49)%、(104.70±4.69)%,土贝母苷甲在1.18～88.5μg/mL浓度范围内线性良好。每1g制剂含土贝母苷甲995.3μg,灌胃给予甲肿消后大鼠血清中土贝母苷甲浓度为3.19μg/mL。结论本方法快速、简便、准确,可用于测定制剂及其血清中土贝母苷甲的含量。

Objective To establish the determination method of Tubeimoside I in Jiazhongxiao preparation and serum of rat. Methods RP-HPLC/UV method was used as follows： Diamonsil C18 column （250 mm× 4.6 mm, 5 μm） and Easyguard column, methanol-water （65 ： 35） as mobile phase with flow rate of 1.0 mL/min, UV detection at 214 nm. Results The mean recovery rates of Tubeimoside I in preparation and serum of rat were （103.49±3.49）% and （104.70±4.69）% respectively. The linearity of Tubeimoside I was shown in the range of 1.18-88.5 μg/mL. Tubeimoside I in preparation and serum of rat were 995.3 μg/g and 3.19 μg/mL. Conclusion The method was simple, reliable and rapid. It＇s quite suitable to be used in the analysis ofTubeimoside I in diazhongxiao preparation and serum of rat.