双黄消炎片中盐酸小檗碱含量测定

Content Determination of Berberine in Double Yellow Antiinflammatory Tablets

目的建立双黄消炎片中盐酸小檗碱的含量测定方法。方法采用高效液相色谱法,以Diamonnsil C18(4.6mm×250mm,5μm)为色谱柱;乙腈-0.05mol/L磷酸二氢钾(35∶65,用磷酸调节pH值至3.0)作为流动相;检测波长为345nm;流速:0.8mL/min;柱温:30℃。结果盐酸小檗碱在0.0407～0.4479μg范围内样品含量与峰面积呈良好的线性关系;重复性RSD=0.59%(n=5);平均回收率为96.71%,RSD=0.37%(n=5)。结论本方法稳定、可靠、灵敏度高、重复性好,操作简便易行,可用于双黄消炎片的质量控制。

Objective To establish the method to determine the content of berberine in Double Yellow Antiinflammatory Tablets. Methods Using HPLC, with Diamonnsil C18 （4.6 mm × 250mm, 5 μm） as column, acetonin-ile-0.05 mol/L potassium dihydrogen phosphate （35 ： 65, phosphate regulation pH value to 3.0） as mobile phase, detection wavelength was at 345mm, flow rate was 0.8 mL/min, and column temperature was 30 ℃. Results In berberine range of 0.040 7-0.447 9 μg, the scope and content of the sample peak area had a good linear relationship, reproducibility of RSD=0.59% （n =5）, and the average recovery rate was 96.71% and RSD=0.37% （n =5）. Conclusion This method is stable, reliable, with high sensitivity and good reproducibility, and easy to put into practice, so it can be used for quality control of Double YeUow Antiinflammatory Tablets.