邪毒壅盛证和气虚证荷瘤小鼠垂体上调与下调的基因

Up and Down-regulated Genes in Pituitary of Tumour Mice of Poisonous Pathogenic Factors Syndrome and Asthenia of Qi Syndrome

目的:揭示荷瘤小鼠早期邪毒壅盛证与气虚证垂体基因表达的差异。方法:采用小鼠标准化四诊及辨证方法,及GeneChip Mouse Exon 1.0 ST Array等技术,检测H22荷瘤小鼠早期邪毒壅盛证和气虚证、中期阳气虚证、中晚期气阴阳虚证3阶段4个证候垂体的基因表达,筛选邪毒壅盛和气虚两证候垂体基因表达与正常比较一致上调或下调的基因。结果:①邪毒壅盛证和气虚证垂体表达上调的基因有5个,其中邪毒壅盛证Alas2、Sgk、Cbln3表达高,十分突出,可能与该证候形成有关;②邪毒壅盛证和气虚证的垂体有13个基因表达几乎关闭,其中Abpg、Pip,以及Car6、Klk1b22、Klk1b3、Klk1b9、Muc10、Ren1、Hspa1a、Wfdc12等基因在正常组表达量大。结论:荷瘤小鼠垂体早期出现显著的衰退表现,而个别基因表达的增加,可能与邪毒壅盛有关。

Objective： To reveal the difference of Gene expression in Pituitary of Tumour Mice of Poisonous Pathogenic Factors Syndrome （PPFS） and Asthenia of Qi Syndrome （AQS）. Methods：Used quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array, pituitary gene expression of normal mice and tumour mice of PRFS ns AQS on earlier period, asthenia of yang and qi syndrome on intermediate stage, asthenia of qi and yin and yang syndrome on middle-late stage were measured. The up and down-regulated genes in pituitary of tumour mice of PRFS and AQS were chosen. Results ： Five genes were up-regulated in pituitary of tumour mice of PRFS and AQS. Among them, Alas2, Sgk and Cbln3 expressed highly, which maybe relative to syndromes. Thirteen genes were nearly closed in pituitary of tumour mice of PRFS and AQS. Most of them expressed highly such as Abpg, Pip, Car6, Klklb3, Klklb9, Muc10, Renl, Hspala, Wfdc12. These genes probably played important roles in normal function in pituitary, whose close-up perhaps concerned with stringent state of tumor mice at earlier stage and result in serious prognosis. Conclusion ： Noticeable declined appearance emerged at earlier stage of tumour mice. The increase of individual genes perhaps related to PRFS.