肝硬化腹水并发白发性细菌性腹膜炎病人血浆和腹水尿激酶型纤溶酶原激活物及其受体水平的分析

Analysis of urokinase-type plasminogen activator and its receptor level in plasma and ascites of cirrhosis with Spontaneous bacterial peritonitis

目的检测肝硬化腹水并发自发性细菌性腹膜炎（SBP）病人血浆和腹水中尿激酶型纤溶酶原激活物（uPA）及其受体（uPAR）的含量变化，分析肝硬化并发自发性细菌性腹膜炎病人血浆和腹水uPA和uPAR水平的变化及其uPA／uPAR比值与其病情转归的关系。方法应用酶联免疫吸附试验（ELISA）检测肝硬化并发SBP病人血浆和腹水23例及正常人30例血浆中尿激酶型纤溶酶原激活物（uPA）及其受体（uPAR）。结果①SBP病人血浆uPA和uPAR含量水平分别为（0．63±0．29）μg/L、（0．47±0．35）μg／L，均明显高于正常对照组（O．05±0．01）μg／L、（0．08±0．009）μ／L，二者相比，有显著差异（P〈0．01）；②SBP病人腹水uPA和uPAR含量分别为（0．68±0．35）μg／L、（0．54±0．42）μg/L，均明显高于其血浆组，有显著差异（P〈0．05）；③SBP病人血浆uPA／uPAR比值分别为（1．93±1．26）μg/L、（1．91±1．33）μg／L均明显高于对照组（0．63±0．07）μg／L，有非常显著差异（P〈0．01）；但其血浆和腹水之间uPA／uPAR比值相比较差异没有统计学意义（P〉0．05）。结论肝硬化腹水SBP病人腹水中uPA及其受体uPAR含量水平高于其血浆中的水平，血浆和腹水uPA／uPAR比值在评估肝硬化病人的病情转归方面有很高的临床价值。

Objective To exam the urokinase-type plasminogen activator （uPA） and its receptor （uPAR） levels in plasma and ascites of hepatocirrhosis with Spontaneous bacterial peritonitis （SBP）, and study the relations between uPA and uPAR levels and uPA/uPAR ratio change with disease outcome. Methods uPA, uPAR was detected by Enzyme-Linked Immunosorbnent Assay （ELISA） in 23 cases of cirrhosis with SBP and 20 cases of health peoples, the results were analysed by the statistical method. Results The plasma uPA and uPAR levels in cirrhosis with SBP were （0.63±0.29） μg/L, （0.47±0.35） μg/L, in normal group uPA and uPAR levels were （0.05±0.01） μg/L, （0.08＋0.009）μ g/L, it was obviously higher in cirrhosis with SBP than that of normal group and there was remarkable difference between them（P〈0.05）. Ascites uPA and uPAR of cirrhosis with SBP were （0.68±0.35）μg/L, （0.54±0.42）μg/L, it was also higher in cirrhosis with SBP than that of normal group obviously, and there was remarkable difference between them（ P〈0.05）; cirrhosis with SBP plasma and ascites uPA/uPAR ratio were （1.93±1.26）μg/L, （1.91±1.33）μg/L, obviously higher than that of normal group （0.63±0.07）μg/L, there was remarkable difference （P〈0.01）; But between cirrhosis with SBP patients plasma and ascites uPA/uPAR ratio were no remarkable difference, （P〉0.05）. Conclusions uPA and uPAR levels in ascites of cirrhosis with SBP patients were higher than in plasma, the plasma and ascites uPA/uPAR ratio had clinical significance, in patient＇s outcome.