杜仲细胞悬浮培养生产绿原酸的初步研究

Preliminary study on the cell suspension culture of Eucommia ulmoides and secondary metabolite-chlorogenic acid

对影响杜仲细胞悬浮培养及其次生代谢物绿原酸产生的几种主要因子进行了研究。结果表明,在杜仲细胞悬浮培养生产绿原酸的过程中,第15天绿原酸的含量达到最大值。35g/L的蔗糖为最适碳源,MS培养基为最适悬浮培养基,pH为5.3时利于绿原酸的合成,2,4-D、NAA对绿原酸合成的促进效果不大,添加1.0mg/L的6-BA绿原酸的合成效果较好。

Some major factors affected the cell suspension culture of Eucommia ulmoides and the production of chlorogenic acid were investigated. The results showed that the concentration of chlorogenic acid was the highest on 15th day, sucrose was better than glucose and maltose for cell growth and chlorogenic acid synthesis and the optimum concentration was 35 g/L. The optimal media was MS media and the proper pH value was 5.3. Some exogenous hormones such as 2,4-D and NAA had little effect on chlorogenic acid synthesis, but 1.0 mg/L 6-BA could promote the production of chlorogenic acid.