毛竹叶抑菌活性成分提取工艺研究

Research on Extraction of Anti-microbial Active Components from Phyllostachys pubescens Mazel ex H.de Lehaie Leaves

研究了毛竹叶活性成分的提取工艺及其抑菌效果。用液体培养基稀释法对金黄色葡萄球菌和大肠杆菌进行了最低抑菌浓度(M IC)测定。结果表明,温度60℃、乙醇体积分数60%、液固比20∶1(mL∶g)、提取2 h条件下毛竹叶提取物对金黄色葡萄球菌和大肠杆菌有很强的抑制作用,对金黄色葡萄球菌的M IC为3.125 g/L,对大肠杆菌的M IC为6.25 g/L。

Extraction of active components from moso bamboo （Phyllostachys pubescens Mazel ex H. de Lehaie） leaves and their anti-microbial actives were studied. The minimum inhibitory concentration （MIC） of the active components from P. pubescens leaves against Staphylococcus aureus and Escherichia coli was determined by serial liquid dilution method. The results showed that anti-microbial effect of extracts from P. pubescens leaves against S. aureus and E. coli was optimum with the extraction condition： 60 ℃, 60 % （ volume fraction） ethanol, ratio of liquid to solid 20：1,2 h. The MIC of the extracts from P. pubescens leaves was 3. 125 g/L against S. aureus, and 6.25 g/L against E. coli.