低剂量脉冲超声波对培养细胞增殖作用

Effect of low intensity pulse ultrasound on proliferation of human skin fibroblasts

目的研究不同条件低剂量脉冲超声波刺激体外培养人皮肤成纤维细胞的增殖情况。方法采用5-溴脱氧尿苷(BrdU)掺入法测定成纤维细胞增殖情况。结果在BrdU掺入法测定中,细胞培养液不含小牛血清(FCS)时,超声波刺激时间为8和14 min剂量组的BrdU阳性着色细胞率与对照组比较有显著性增高(P<0.05,P<0.01);而细胞培养液含10%FCS时,超声波刺激时间为5,8,11和14 min剂量组的BrdU阳性着色细胞率与对照组比较,差异有统计学意义(P<0.01)。且培养液的FCS含量分别为0%,5%和10%时,BrdU阳性着色细胞率的高峰值均在低剂量脉冲超声波刺激时间为8～11 min时。结论在一定刺激时间内,低剂量脉冲超声波能促进人皮肤成纤维细胞增殖,其增殖情况与超声波的刺激时间以及细胞培养液中是否含有小牛血清有关。

Objective To determine whether low intensity pulse ultrasound has any effect on cell proliferation of human skin fibroblasts in vitro. Methods The proliferation effects of human skin fibroblasts were determined by BrdU incorporation assays. Results The proliferation of human fibroblasts was determined after 5, 8, 11, and 14 minutes of low intensity pulse ultrasound treatment. In cell culture without FCS DMEM medium, there was a significant increase of stained cell num- ber after 8 and 14 minutes of treatment（P 〈： 0.05 and P 〈 0.01, respectively）. In the cell culture with 10 % FCS DMEM medium, there was a significant increase of stained cell number after 5, 8, 11 and 14 minute treatment（ P 〈 0.01 ）. Conclusion Low intensity pulse ultrasound has stimulation effect on cell proliferauon of human skin fibroblasts in vitro. In addition, the proliferation effect can be affected by concentration of FCS in the culture medium and the treatment time.