血清G—CSF检测及在儿童感染中应用的研究

Study on Detection of Serum G-CSF and It's Application in Infection of Children

采用酶联免疫吸附试验（ELISA）等方法检测了119例儿童感染患者和39例正常健康儿童血清粒细胞集落刺激因子（G－CSF）水平，并作两种方法的相关性比较。结果发现119例儿童感染患者ELISA法检出阳性事为70.9％（84／119）．生物学方法检出阳性率为75.6％（90／119）。正常健康儿童血清G－CSF检出阳性率为15．6（5／39）。儿童感染患者G-XSF检出阳性率显著高于正常对照组（P＜0．01）。两种方法相关性分析显示有显著相关（r=0.84）。结果表明血清G-CSF检测可作为儿童感染诊断的一种辅助指标。

The levels of serunl G-CSF in 119 cases infection of cbildren and in 39 cases healthchi ldren was detected by ELISA and biologic methods. Statistical relation of the two assays was compared. Our finding showed that postive rate of serum G-CSF was 70. 5 percent (84/119) by ELISA, 75. 6percent (90/119) by biologic method in infection of children and 5. 1 percent (2/39) by ELISA, 15. 6percent (5 /39) by biologic method in health children. The postive rate in infection ofchildren was markedly higber than that of control groups (P<0. 01). The correction analysisshowed that tbe two method was signficantly related (r=0. 84). It was suggested that detectionof serum G-CSF may be a sensitive indicator of dignosis of infection of children.