用套式PCR诊断肺炎支原体感染

Detection of Mycoplasma Pneumoniae by using the nested Polymerase Chain Reaction

应用套式PCR检测患儿咽部分泌物中的肺炎支原体（MP），阳性率为44．4％（28／63），比单式明显增高，由于两次扩增，增强信息量，增除生殖道支原体的假阳性，排除了其他干扰，提高检测的特异性、敏感性。

We have used the allele specific nested polymerase chain reaction to detect Mycoplasmapneumoniae from patient's throat swab samples, The positive rate is 49. 3%(28/63), aud is significantlybigher thau by Common PCR method. Because of being twice amplified, it contaius more iuformation, getsrid of the false positive of Mycoplasma genitalium, eliminates other interfereuces, and rises it'sspecificity and sensitivity in detectiug Mycoplasma, pneumoniae.