摘要
为了进一步提高姬松茸胞内多糖得率,采用单因素实验和响应曲面法,对姬松茸菌丝体胞内多糖碱提取工艺进行了研究。结果表明优化的碱提取工艺为提取时间4.63 h,碱液浓度1.03 mol/L,提取温度60.90℃,液料比85.85 mL/g,在此条件下胞内多糖的提取得率为(273.49±1.59)mg/g干菌体,明显高于水提取所得到的胞内多糖得率。这为姬松茸碱提胞内多糖理化性质、生理活性等方面进一步的研究提供切实可行的高效提取方法。
Alkaline extracting of intracellular polysaccharides (IPS) from Agaricus blazei Murill was investigated using response surface methodology. The results showed that the optimal extracting process conditions are as follows: extraction time 4.63 h, base concentration 1.03 mol/L , temperature 60.90 ℃, liquid to solid ratio 85.85 mL/g. Under the optimal conditions, the extracting ratio of crude IPS from Agaricus blazei Murill was (273.49 ± 1.59) mg/g dry mycelium. The method is better than that of water extracting and futher study of the characteristics of IPS is effective.
出处
《生物加工过程》
CAS
CSCD
2008年第5期21-26,共6页
Chinese Journal of Bioprocess Engineering
基金
国家863计划资助项目(2007AA10Z346)
关键词
姬松茸
提取
多糖
响应曲面法
液体发酵
Agaricus blazei Murrill
extracting
polysaceharide
response surface methodology
submerged culture