姬松茸胞内多糖碱提取工艺

Alkaline extraction process of intracellular polysaccharides from Agaricus blazei Murill

为了进一步提高姬松茸胞内多糖得率,采用单因素实验和响应曲面法,对姬松茸菌丝体胞内多糖碱提取工艺进行了研究。结果表明优化的碱提取工艺为提取时间4.63 h,碱液浓度1.03 mol/L,提取温度60.90℃,液料比85.85 mL/g,在此条件下胞内多糖的提取得率为(273.49±1.59)mg/g干菌体,明显高于水提取所得到的胞内多糖得率。这为姬松茸碱提胞内多糖理化性质、生理活性等方面进一步的研究提供切实可行的高效提取方法。

Alkaline extracting of intracellular polysaccharides （IPS） from Agaricus blazei Murill was investigated using response surface methodology. The results showed that the optimal extracting process conditions are as follows： extraction time 4.63 h, base concentration 1.03 mol/L , temperature 60.90 ℃, liquid to solid ratio 85.85 mL/g. Under the optimal conditions, the extracting ratio of crude IPS from Agaricus blazei Murill was （273.49 ± 1.59） mg/g dry mycelium. The method is better than that of water extracting and futher study of the characteristics of IPS is effective.