摘要
目的建立用高效液相色谱法测定酒石酸溴莫尼定滴眼液含量的方法。方法采用AgilentC18柱。以磷酸盐缓冲液（取磷酸二氢钠7．8g，十二烷基磺酸钠0．41g与三乙胺1．0mL，加水至1000mL，摇匀）-乙腈（3：1）为流动相；检测波长为245nm；流速为1．0mL·min^-1。结果酒石酸溴莫尼定浓度在5～50μg·mL^-1范围内与峰面积呈良好的线性关系（r=0．9999）；最低检测限为0．10ng；平均回收率为99．93％，RSD为0．308％。结论此法可用于测定酒石酸溴莫尼定的含量。
OBJECTIVE To establish an HPLC method for the determination of brimonidine tartrate in Brimonidine Tartrate Eye Drops. METHODS Analysised by RP--HPLC,an agilent C18 column was used; A mixture of acetonitrile--phosphate buffer solution（sodium dihydrogen phosphate 7.8g, sodium laurye sulfonate 0.41g and triethylamine 1mL, dilute to 1000mL with water）（3 ： 1）was used as the mobile phase. The detection wavelength was 245nm The flow rate was 1.0mL · min^-1. RESULTS The detection limit was 0.10ng（245nm）. The standard curve was linear in the range of 5 to 50μg · mL^-1 （ r =0. 9999）. The average recovery was 99.93% and RSD was 0. 308%. CONCLUSION The method was suitable for determining the content of Brimonidine Tartrate Eye Drops.
出处
《齐鲁药事》
2008年第10期592-593,共2页
qilu pharmaceutical affairs
