慢性HBV感染者外周血单个核细胞干扰素-γ的表达及其影响因素

The expression of interferon-γ in peripheral blood monocytes in patients with chronic HBV infection and its influencing factors

目的探讨慢性乙肝病毒(HBV)感染者干扰素-γ(IFN-γ)的表达水平及其影响因素。方法采用实时定量RT-PCR技术和ELISA法分别检测慢性HBV感染者外周血单个核细胞(PBMCs)中IFN-γmRNA的表达和血清IFN-γ的分泌水平;巢式PCR技术检测PBMCs中HBV DNA。结果慢性HBV感染者IFN-γ的表达和分泌水平均明显低于对照组(P<0.01,P<0.05);慢性HBV携带者IFN-γ的表达和分泌水平均显著低于HBeAg阳性组(P均<0.05)和HBeAg阴性组(P均<0.01);HBeAg阳性组IFN-γ的表达和分泌水平均明显低于HBeAg阴性组(P均<0.01);PBMCs中HBV DNA阳性组IFN-γ的表达和分泌水平均明显低于HBV DNA阴性组(P均<0.01);IFN-γ表达和分泌水平与血清HBV DNA水平均呈负相关(P均<0.01)。结论慢性HBV感染者IFN-γ低表达;高病毒载量可抑制IFN-γ的表达。

Objective To study the expression of interferon-γ（IFN-γ） in peripheral blood monocytes（PBMCs） in patients with chronic HBV infection and factors that may affect its expression.Methods The expression of IFN-γ mRNA and secretion of IFN-γ in chronic HBV infectious patients were determined by real-time RT-PCR and ELISA,respectively;HBV DNA in PBMCs was detected by nested-PCR. Results The expression and secretion of IFN-γ were all significantly lower in chronic HBV infectious patients than in controls（P 〈0.01,P 〈0.05）.The expression and secretion of IFN-γ were all obviously lower in chronic HBV carriers than in HBeAg positive group（P 〈0.05,P 〈0.05） and HBeAg negative group（P 〈0.01,P 〈0.01）.The expression and serection of IFN-γ were all significantly lower in HBeAg positive group than in HBeAg negative group（P 〈0.01,P 〈0.01）.The expression and secretion of IFN-γ showed significantly lower level in HBV DNA from PBMCs positive group than in HBV DNA negative group（P 〈0.01,P 〈0.01）;the expression and secretion of IFN-γ were negatively correlated with the amounts of HBV DNA in serum（P〈0.01,P〈0.001）. Conclusion The expression of IFN-γ in chronic HBV infectious patients was lower;high amounts of HBV DNA may inhibit the expression of IFN-γ.