重型颅脑损伤后氧自由基活动与脑水肿、脑电图和超微结构变化关系的实验研究

The Relation between the Changes of EEG and Ultrastructuresof Brain and the Activity of Oxygen Free Radicals in Traumatic Brain Edema in Cat

采用硬膜外自由落体打击法致猫重型颅脑损伤,随机分为外伤组、F-SOD治疗组和L-SOD治疗组,观察伤后1,3,6,24 h动物意识活动情况、EEG、血清和CSF中LPO和SOD含量、24h后脑含水量、脑组织LPO和SOD含量和超微结构变化,并探讨各观察指标之间的关系.结果显示:脑内OFR的形成是脑外伤后继发性脑损伤和脑水肿的关键因素之一,观察EEG的变化能在一定程度上反映脑内OFR的产生情况.并对其有关机理进行了探讨.

An animal model of severe traumatic brain edema induced by extradural impact wasestablished in cat. Then the animals were divided into three groups randomly: injury group, free-SOD (F-SOD) treated group and liposome entrapped SLD (L-SOD) treated group. The examina-tion indications include conciousness score, EEG, LPL and SOD in blood serum and in cere-brospinal fluid at 1,3, 6, 24 hours after the injury and the water content, LPO and SOD, and theultrastructure changes of the brain at the 24 hours after injury. The relation between the changesof EEG and ultrastructures of brain and the activity of oxygen free radicals are also observed. Theresults showed that formation of oxygen free radicals is one of the main facts of the delayed devel-opment of the traumatic brain edema and that the changes of EEG can mirro the production of theoxygen free radicals in the brain. The relative machnisms are also discussed in this paper.