E838联合γ射线对淋巴瘤荷瘤小鼠的协同抑瘤作用

Antitumor Effects of Combination Treatment of E838 with γ-ray for Mouse Lymphoma

目的本研究主要观察E838对小鼠移植性淋巴瘤的体内抑瘤效果及相关的生物学指标，探讨合用^137Csγ射线是否具有抑瘤增效作用。方法取2～3mm^3淋巴瘤瘤块接种于IRM-2小鼠腋部皮下，24h后将荷瘤小鼠随机分为对照组、单放组、E838低、中、高药物组及药物合用照射组、环磷酰胺组。药物组与药物合用照射组对应性腹腔注射相同剂量E838，每日1次，连续7天，环磷酰胺隔日1次×4。合用照射组于给药的第4天进行全身10y照射，每日1次，连续5天。观察各组小鼠骨髓有核细胞数和肿瘤抑制率。结果E8383个剂量对小鼠移植性淋巴细胞瘤的抑瘤率分别为（44．14±15．96）％、（70．74±11．17）％和（50．00±18．09）％，与对照组比较差异有统计学意义（P〈0．001），骨髓有核细胞数与对照组相比则明显提高。E838合用^137Csγ射线能提高抑瘤效果，抑瘤率分别为（65．43±2．13）％、（77．13±6．38）％和（67．55±11．17）％，（P〈0．001），对肿瘤的杀伤作用高于单放组和单药治疗组。结论E838对小鼠肿瘤细胞具有良好的抑制作用，E838合用γ射线具有协同抑瘤作用，在适当剂量范围内可以促进荷瘤小鼠放疗后骨髓损伤修复。

Objective To investigate the tumor inhibitory effects of E838 and the combined antitumor effects of EB38 and ^137Cs y-ray irradiation. Methods IRM-2 mice transplanted with 2-3 mm^3 lymphoma（LM） tissue for 24h, were randomized into nine groups： control group, radiation group, high, middle, low EB38 dose group, different dose FA38 combined with radiation group and cyclophosphamide group. E838 group and combined group administered with same dose EB38 daily for 7 days, cyclophosphamide group administered every other day 4 times. Combined group radiated with 1 Gy/day for 5 days after the mice wereadministered with E838 for 4 days The size of LM tumor and the bone marrow cells of different group were measured. Results The mouse LM tumor inhibitory ratios of three E838 doses groups were （44. 14 ± 15. 96）%, （70. 74 ± 11. 17）% and （50. 00 ± 18.09） % respectively. There was a significant difference between E838 groups and control group （19 〈0. 001）. The level of bone marrow cells counting was markedly elevated. E838 combined with 137Csγ-ray could enhance tumor inhibitory effect, the tumor inhibitory ratios of three combined groups were （65. 43 ±2. 13 ） %, （77. 13 ± 6. 38 ） % and （67. 55 ± 11.17） %, there were obviously increased compare with control and radiation group respectively （P〈0. 001 ）. Conclusion E838 has good tumor inhibitory effects on lymphoma transplanted mice tumor. The synergistic antitumor effects was found after the mice were treated with E838 and γ-ray irradiation, the bone marrow function recovery after irradiation was speed up when the mice were administrate with E838 of some definite doses.