原发性胆汁性肝硬化患者外周血单个核细胞中Toll样受体3mRNA表达水平的研究

The expression of Toll-like receptors-3 mRNA on peripheral blood monocytes in patients with primary biliary cirrhosis

目的探讨原发性胆汁性肝硬化（PBC）患者外周血单个核细胞（PBMCs）中Toll样受体3（TLR3 mRNA）表达及其与疾病活动性的关系。方法利用实时荧光定量-反转录-聚合酶链反应（FQ—RT—PCR）的方法，检测55例PBC患者（活动期33例、缓解期22例）、20例肝癌患者（疾病对照组）和24名健康体检者（健康对照组）PBMCs中TLR3 mRNA的表达水平。以△Ct=Ct（待测基因）-Ct（内参基因）来比较基凶表达水平的高低。结果PBC患者活动期TLR3 mRNA的表达水平高于疾病缓解期，差异有统计学意义（P〈0．05）；同时也高于肝癌患者和健康对照组，差异有统计学意义（P〈0．05）；缓解期TLR3 mRNA的表达水平与健康人和肝癌对照组差异无统计学意义（P〉0．05）。结论PBC患者TLR3 mRNA表达异常，可能参与了PBC的发病过程并与疾病的活动性有关。

Objective To explore the relationship of Toll-like receptors （TLR）-3 mRNA expression level on peripheral blood monocytes （PBMCs） with disease activity of PBC in patients with primary biliary cirrhosis （PBC）. Methods The expression level of TLR-3 mRNA on peripheral blood monocytes was tested in 55 PBC cases （33 cases in active phase, 22 cases in stable stage）, 20 cases with hepatoma （as disease controls） and 24 healthy controls. By real-time quantitative reverse transcription polymerase chain reaction （FQ-RT-PCR） methods. β-actin was selected as the internal control. △Ct=Ct （target gene）-Ct（internal reference gene） were used to measure the gene expression level. Results The mean TLR-3 mRNA expression of PBC in active stage was higher than that of stable stage, hepatoma （P〈0.001） as well as normals. The difference was significant （P=0.011〈0.05）. The mean TLR-3 mRNA was not different than that of the stable stage （P=0.221〉0.05） and normal controls （P=0.347〉0.05）. There was no difference between patients with stable PBC and normal controls （P=0.590〉0.05）. Conclusion The TLR-3 mRNA expression level of PBMCs is elevated in active PBC patients. It may correlate with the pathogenesis and disease activity of PBC.