卡培他滨对食管癌细胞系EC9706细胞毒性和放射增敏作用的研究

Cytotoxic effect and radiosensitivity of capecitabine on esophageal carcinoma cell line EC9706

目的:探讨卡培他滨对食管癌细胞系EC9706细胞毒性和放射增敏作用。方法:取健康成年家兔1只,以卡培他滨141mg/kg,对其进行灌胃,分别于灌胃前、后2.5～3h,取耳缘静脉血8～10mL,不加抗凝剂,并防止溶血和细菌污染,离心取血清-20℃保存备用;用CCK8分别检测不同量的加药血清和对照血清对食管癌细胞系EC9706细胞毒性,同时观察不同量的加药血清对食管癌细胞系EC9706的放射增敏作用。结果:加药血清对食管癌细胞系EC9706细胞有明显的抑制作用,其抑制作用48h达到高峰,2、4、6、8和10μL血清作用细胞48h后抑制率分别为14.29%、23.96%、50.59%、52.16%和53.96%(P均为0.00),72h其细胞毒性无增加;加药血清孵育2.5h后,在相同放射剂量作用下,不同量血清组的OD值均显著低于对照组(P值均为0.00),其增敏作用无明显的剂量依赖性。结论:卡培他滨对食管癌细胞系EC9706有明显的细胞毒性和放射增敏作用。

OBJECTIVE： To investigate the cytotoxic and radiosensitizing effect of capecitabine on esophageal carcinoma cell line EC9706. METHODS： One healthy adult rabbit was treated with oral administration of capecitabine in a dose of 141 mg/kg, amd before and after 2.5 3 hours of that, 8--10 mL blood was drew from the super ficial vein of the ear respectively, being no anticoagulant, avoiding hemolysis and infection, and the serum was collected after centrifuged, and it was reserved at --20 ℃. Cytotoxic effects of different doses of the serum on EC9706 cell line were evaludted respectively by using CCK 8 assay, and the radiosensitizing effects of different doses of the serum combining with different doses of irradiation were evaludted by using the same way. RESULTS： The serum treated with capecitabine obviously restrained EC9706 cell line. The peak of the inhibitory rate was at 48 hours. Being exposed to 2,4,6,8,10 μL of the serum, the inhibitory rates of the cell growth were 14.3%, 23.0%, 50.6%, 52.2% and 54.0% respeitively （P=0.00）, The serum showed no more inhibitory effect at 72 hours. Under the same irradiation, OD of EC9706 cell line exposed to different doses of the serum treated with eapecitabine was obviously lower than that of the control group （P= 0.00）, but the the radiosensitizing effect did not depent on the doses of the serum treated with capecitabine. CONCLUSION： Capecitabine has a significant cytotoxic and radiosensitizing effect on esophageal carcinoma cell line EC9706.