藤茶双氢杨梅树皮素诱导人肺癌A549细胞凋亡及其机制研究

Study on the effect and mechanism of ampelopsin-inducing apoptosis on human lung cancer A549 cell

目的:探讨藤茶双氢杨梅树皮素(ampelopsin,APS)抗人肺癌A549细胞的作用。方法:以MTT法和集落形成法观察APS对人肺癌A549细胞的体外抑制作用;采用RT-PCR技术测定APS对人肺癌细胞株A549抑瘤基因p53表达的影响。结果:APS能明显抑制体外培养的A549细胞的生长,MTT法中IC50为9.8μg·mL-1;细胞生长曲线法提示其对A549细胞的生长也有明显的抑制作用;集落形成试验中,当药物浓度为14.84,9.90,6.60,4.40μg·mL-1时,抑制率分别为100%,91.6%,64.5%和23.4%;当浓度为25μg·mL-1时,APS能显著增强p53基因的表达水平。结论:APS对体外培养的人肺癌A549细胞的生长有明显的抑制作用,对抑癌基因p53表达的影响是其抗肿瘤的作用机制之一。

Objective：To investigate the anti-tumor effect of ampelopsin（APS） in vitro.Methods：The growth inhibition was analyzed by MTT and colony forming experiment in the lung cancer A549.The effect of APS on the expression of p53 mRNA in the human lung cancer cell line A549 was analyzed by RT-PCR.Results：Ampelopsin could obviously inhibit the growth of A549 cells cultured in vitro,with IC50 = 9.8 μg · mL^ -1 in MTT assay.The inhibition ratio in colony forming experiments were 100%,91.6%,64.5% and 23.4%,respectively,when dosages were 14.84,9.90,6.60 and 4.40 μg · mL^-1.APS can significantly improve the expression of p53 gene at 25 μg · mL^ -1.Conclusion：Ampelopsin can inhibit the growth of A549 in vitro remarkably,and the effect on expression of p53 gene is one of its anti-tumor mechanisms.