冷应激对雏鸡十二指肠及血清NO代谢的影响

Effects of Cold Stress on NO Metabolism in Duodenum and Serum of Chickens

探讨冷应激对雏鸡十二指肠及血清NO代谢的影响。方法:以健康15日龄雏鸡为试验对象,进行急性和慢性冷应激(12±1)℃处理,检测十二指肠和血清NO含量以及T-NOS和iNOS活性。结果:急性冷应激时十二指肠和血清NO含量、十二指肠T-NOS活性随应激时间的延长呈波动性变化;十二指肠、血清iNOS活性和血清T-NOS活性呈现先升高后降低的变化。慢性冷应激时,随着冷应激时间的延长NO含量先降低后升高,T-NOS活性先升高后降低,iNOS活性有所升高。急性和慢性冷应激可以引发雏鸡十二指肠和血清的NO代谢改变。

The effects of cold stress on NO metabolism in duodenum and serum of chickens was studied. 15-day-age chickens were used as experimental animals. They were subjected to acute and chronic cold stress （12＋1）℃. The NO content,T-NOS activity and iNOS activity in the duodenums and serum were detected. The results showed that during the acute cold stress,NO content in the duodenum and serum and T-NOS activity in the duodenum took on the fluctuating changes,and iNOS activity in the duodenum and serum and T-NOS activity in serum first increased and then decreased. During the chronic cold stress, NO content first decreased and then increased,T-NOS activity first increased and then decreased,and iNOS activity slightly increased. It was demonstrated that acute and chronic cold stress could alter the NO metabolism in the duodenum and serum.