中药制剂对中国对虾免疫活性物的诱导作用

INDUCTION OF IMMUNITY SUBSTANCE IN PENAEUS CHINENSIS BY CHINESE HERBAL MEDICINE

分别于1994和1995年9—10月以胶州湾海捕中国对虾为材料，取其血淋巴测定血凝素活力、溶菌活力，并以投喂添加Vc,VB6，中药制剂1号，中药制剂2号的药饵17d、25d后的免疫活性物的活力动态变化，及注射1号、2号中药制剂3d后的活力变化等为指标，权衡＿肝述物质激发中国对虾免疫系统的功能。实验结果表明，1号中药制剂能激发中国对虾免疫系统的功能，诱导血凝素活力，溶菌活力升高，可以提高机体非特异性体液免疫功能。2号中药制剂抑制或破坏中国对虾非特异性体液免疫系统的功能，导致血凝素活力、溶菌活力降低，直至对虾死亡。

The shrimp (Penaeus chinensis), being 11-13cm in body length and 15-20g in body weight, was collected from Jiaozhou Bay in September-October 1994 and 1995, respectively, and then cultllred in laboratory tank. The shrimps were kept in the tank (100×75×80cm3) for 7-10 days before the experiment and 2 / 3 of aerated seawater were changed daily, and were fed with diet supplemented with Pollen,Vc VB6 and Np. 1 or No. 2 (Tab. 1) chinese herbal medicine respectively, another group were injected with No. 1 or No. 2 chinese herbal medicine. The haemolymph was taken from the fed shrimps, which were cultured for 17 or 25 days, and the injected shrimps which were cultured for 3 days to test change of their nonspecific immunity, i. e. bacteriolytic activity and agglutinative achvity. The results were as followsi1. When the shrimps were fed with diet containing Pollen, V.c VB6 their nonspeciflc immunity, i.e. bactenolytic activity and agglutinative activity could not be irnproved (Tab.2). 2. The bacteriolytic activity in the serum was, respectively, 0.167 and 0.73 for the shrimps fed with diet containing No. 1 chinese herbal medicine, measured for 17 and 25 days after feeding, it was 0.135 and 0 for the shrimps fed with diet contaning No.2 chinese herbal medicine measured on the 17 and 25 days, respectively (Tab.3).3. The agglutinative activity in the serum was, respectively, 64 and 128 for the shrimps fed with diet containing No. 1 chinese herbal medicine, measured for 17 and 25 days after feeding, it was 32 and 8 for the shrimps fed with diet containing No.2chinese herbal medicine measured on the 17 and 25 days, respectively (Tab.3).4. The bacteriolytic activity in the serum was 0.65 and agglutinative achvity titer in the serum was 64, for the shrirnps, which were injected 100μl No. 1 chinese herbal medicine 3 days latter. The bacteriolyhc achvity in the seruxn was 0, and agglutinative activity hter in the serum was 4, for the shrimps, which were injected 100μl No.2 chinese herbal medicine 3 days later (Tab.4).5. The colour of haemolymph was blue for the shrimps fed with diet contaning No. 1 chinese herbal medicine for 17 and 25 days, it was same as the control (Tab.3).The shrimps were injected with 100μl chinese herbal medicine 3 days later, the haemolymph colour was same as the control (Tab.4). The colour of haemolymph was blue for shrimps fed with diet contalning No. 2 chinese herbal medicine for 17 days,but the haemolymph colour was orange for the shrimps fed with diet containing No.2chinese herbal medicine for 25 days (Tab.3). The shrimps were injected with 100μl No. 2 chinese herbal medicine 3 days later, and the haemolymph colour was also orange (Tab.4).The above results showed that No. 1 chinese herbal medicine could improve nonspecific immunity of the shrimps, and increase their disease resistance.