摘要
目的建立测定青蒿琥酯的含量测定方法。方法采用反相高效液相色谱-柱后衍生化法,流动相为乙腈-醋酸盐缓冲液(pH4.0)(60∶40),流速0.8ml/min;衍生试剂为1mol/L氢氧化钾的90%乙醇溶液,流速0.5ml/min;反应温度70℃,柱温30℃,检测波长289nm。结果青蒿琥酯在2～100μg/ml范围内线性关系良好,以峰面积对青蒿琥酯浓度进行线性回归,方程为A=1746.8+8525.4C,r=0.99999;日内、日间精密度RSD均<2%;平均回收率均在98.0%～102.0%。结论该法准确、简便、重现性好,可用于青蒿琥酯的质量控制。
Objective To establish a method for determination of artesunate by HPLC post-column derivation. Methods High performance liquid chromatography （HPLC） was used, with the mobile phase consisting of acetonitrile and pH 4.0 HAc/NaAc buffer （60：40） delivered at a flow-rate of 0.8 ml/min and 1 mol/L KOH in 90% ethanol as post-column reagent delivered at a flow-rate of 0.5 ml/min. The temperature of reaction was set at 70 ℃ and the column temperature was maintained at 30 ℃. Wavelength of detection was set to 289 nm. Results The linear range ofartesunate was 2- 100 μg/ml. Linear regression between the peak area and artesunate followed the equation A=1746.8＋8525.4C,r=0.999 99. The inter-day and intra-day coefficient of variation were less than 2 %. Mean recovery was 98.0%-102.0%. Conclusion An accurate, easy and performance-stable method for quality control of artesunate was developed.
出处
《中国药物与临床》
CAS
2008年第11期855-857,共3页
Chinese Remedies & Clinics
基金
国家自然科学基金资助项目(30572367)
