高效液相色谱手性固定相法拆分重酒石酸去甲肾上腺素对映体

Enantiomer Seperation of Norepinephrine Bitartrate with Chiral HPLC method

采用Chiralpak AD-H手性色谱柱在正相条件下直接拆分了重酒石酸去甲肾上腺素对映体,得到优化的色谱条件为:流动相组成为V(正己烷)∶V(乙醇)∶V(乙醇胺)=800∶200∶2,流速为0.8 mL/min。检测条件为UV280 nm。对色谱方法的准确性、线性关系、精确性、检测限等进行了考察。结果表明,方法具有准确度高、线性好、灵敏度高等优点。并对多个样品的比旋光度和光学纯度进行了比较。

An isocratic chiral HPLC method was developed for the separation of Norepinephrine Bitartrate enantiomers. The enantioseperation was carried out on a Chiralpak AD-H column with V （n-hexane）： V（ ethanol） ： V（ethanolamine） =80：20：0. 2 as the mobile phase at UV 280 nm. The method is precise, linear, sensitive and accurate for quantification or ee （%） determination. The relationship between specific rotation and enantiomeric excess purity was studied for Norepinephrine Bitartrate products.