消痰散结方对小鼠胃移植瘤及瘤旁胃组织中白细胞介素6表达的影响

Effect of Xiaotan Sanjie Decoction on the expression of IL-6 in mice with gastric tansplanted tumor and gastric tissues beside tumor

[目的]观察消痰散结方对小鼠胃移植瘤及瘤旁胃组织中白细胞介素6(IL-6)mRNA表达的调节作用,探讨消痰散结方抑瘤、防复发的部分作用机制。[方法]50只昆明小鼠随机分为正常组、0.85%氯化钠组、清热解毒方组、喃氟啶组、消痰散结方组;除正常组外,其余各组通过移植S180瘤块,建立小鼠胃移植瘤模型;接种48 h后各荷瘤组灌胃给药,正常组同期、同条件下一般饮食饲养,观察小鼠生长情况;3周后剥取肿瘤,称取瘤重,计算抑瘤率,瘤及瘤旁胃组织行病理组织学检测,运用RT-PCR方法检测瘤及瘤旁胃组织中IL-6 mRNA表达。[结果]正常胃组织-瘤旁胃组织-瘤组织中IL-6表达呈递增趋势,消痰散结方较清热解毒方、喃氟啶明显抑制肿瘤生长、下调肿瘤及瘤旁胃组织中IL-6 mRNA的表达。[结论]消痰散结方明显下调肿瘤及瘤旁胃组织中IL-6的表达可能是其抑瘤、防复发的机制之一。

[Objeetive]To discover the mechanism of restraining the growth of tumor and preventing turnor recurrence of Xiaotan Sanjie Decoction by detecting the expression of IL-6 in mice with gastric tansplantation tumor and gastric tissue beside tumor, [Methods]Fifty Kunming mice had been randomly divided into normal group,NS group, heat-clearing and detoxicating Decoction group,FT-207 group and Xiaotan Sanjie Decoction group. The mice model of gastric tansplanta- tion tumor was estabilshed except normal group through transplanting S180 tumor block. The mice were given intragastric administration 48 hours after the surgery. Normal mice were feed with general food and drink in the same time and condition. The experiment was terminated 3 weeks later. The state of growth and pathologic character of tumor and gastric tissues beside tumor were observed. RT-PCR was used to detect the expression of IL-6 mRNA. [Results]The expression of IL-6 increased progressively in normal gastric tissue, gastric tissues beside tumor and tumor. Xiaotan Sanjie Decoction can significantly down-regulate the expression of IL-6 more than heat-clearing and detoxicating Decoction and FT-207. [Conclusion] It might be one of the mechanisms of restraining the growth of tumor and preventing recurrence of Xiaotan Sanjie Decoction to down-regulate the expression of IL-6 in tumor and gastric tissue beside tumor.