肝康Ⅳ号对大鼠酒精性脂肪肝的保护作用

Protective effect of Gankang Ⅳ on alcoholic fatty liver in rats

[目的]探讨肝康Ⅳ号对大鼠酒精性脂肪肝的保护作用。[方法]采用高脂饮食和白酒诱导大鼠酒精性脂肪肝模型,以不同剂量肝康Ⅳ号煎液灌胃治疗(治疗组),于8周末处死鼠,采血检测血清天冬氨酸转氨酶(AST)、丙氨酸氨基转移酶(ALT)及肝脏总胆固醇(TC)、三酰甘油(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平,肉眼和显微镜下观察肝组织病理学变化及脂肪变程度计分,并与空白对照组、模型组和东宝肝泰组比较。[结果]模型组肝组织明显脂肪变性,治疗组和东宝肝泰组可显著降低血清AST、ALT和肝组织TC、TG、MDA水平,提高肝组织SOD活性,肝康Ⅳ号治疗效果优于东宝肝泰;肝组织病理检查显示,肝康Ⅳ号可减轻肝组织脂肪变和点灶样坏死,镜下计分显示肝康Ⅳ号中、高剂量组对肝组织改善优于东宝肝泰组。[结论]肝康Ⅳ号可对抗氧化应激和脂质过氧化损害,对大鼠酒精性脂肪肝有良好的治疗效果。

[Objective]To explore the protective effect of Gankang Ⅳ on alcoholic fatty liver in rats. [Methods]Rats were given high fat diet and alcohol to establish model of alcoholic fatty liver and treated with different dosage of Gankang Ⅳ by gastrogavage. The rats were sacrificed at the end of 8th week, the level of aspartate transaminase（AST）, alanine transaminase（ALT） in serum and total cholesterol （TC）, triglyceride （TG）, superoxide dismutase （SOD）, malondialdehyde （MDA） in liver were detected respectively. The liver histopathology was observed and scored by eye and under microscope. The treated groups were compared with the normal control group, model group and Dongbaogantai （DBGT） group. [Results]In model group, the fatty degeneration was obvious. Gankang Ⅳ groups and DBGT group could significantly reduce the level of AST, ALT in serum and the content level of TC,TG,MDA in liver,elevate the activities of SOD in liver. Gankang Ⅳ was more efficient than DBGT on alcoholic fatty liver in rats. Liver histopathology examination showed Gankang Ⅳ could alleviate the fatty degeneration and punctiform necrosis. The fatty degeneration score under microscope showed middle and high dosage of Gankang Ⅳ imporved liver tissue better than DBC-T.[Conclusion]Gankang IV can resist oxidateive stress and lipid peroxidation damage,has good therapeutic efficacy on alcoholic fatty liver in rats.