磁力牵拉对骨增长影响的动物实验模型设计

Effect of magnetic pulling on bone growth of animal models

背景:自由状态下实现运动性牵拉对骨骼变化趋势的影响研究较为少见,归纳其原因是求证方法困难。进行其相关研究最重要的是设计实现自然状态下动态牵拉动物实验模型系统。目的:建立一种新型的、自然状态下的、动态磁力牵拉动物实验模型系统,分析自然运动牵拉力下骨骼应力性变化趋势。设计、时间及地点:分组对照观察,于2002-06/2003-06在河北科技师范学院动物科学系动物实验室完成。材料:7周龄近交系日本大耳白兔24只,体质量(2.5±0.3)kg,雌雄不限。永磁片16枚由沈阳钕铁硼永磁材料有限公司提供。方法:利用实验动物、磁体、粘扣和铁质垫板等器材建立了"磁力牵拉对骨增长影响的动物实验模型系统"。将24只兔分为3组,每组8只。牵拉组采用磁力牵拉系统,放在有铁质垫板的饲养笼内饲养。磁力组仅将磁片固定于兔子右后腿脚底部,将兔放在有铁质垫板的饲养笼内饲养。对照组不附加任何装置放入普通竹条垫板笼饲养。主要观察指标:各组兔经不同处理4个月胫骨和股骨增长变化。结果:各组兔分别处理4个月时,与对照组比较,牵拉组胫骨增长2.61mm,股骨增长1.56mm,磁力组胫骨增长0.728mm,股骨增长0.41mm,差异均有显著性意义(P<0.01)。结论:"模型系统"在可控制实验条件下模拟实现了自然运动状态下骨磁力牵拉全过程,且成本低、操作便捷、重复性强,能够满足运动牵拉对骨骼影响方面研究的实际需要。

BACKGROUND： It is rare to study the impact on the changes of skeletons by exercise-induced pulling under the state of freedom, and the reasons may be that the method of verification is difficult. The related researches should focus on achieving the dynamic pulling traction in animal models at natural state. OBJECTIVE： To establish an animal model system of dynamic magnetic pulling at natural conditions, and to analyze the changes of skeletal stress under the state of natural pulling. DESIGN, TIME AND SETTING： Control group observations were finished in the laboratory of Animal Science Department, Hebei Normal University of Science and Technology from June 2002 to June 2003. MATERIALS： Twenty-four Japanese inbred rabbits aged 7-weeks old, weighing （2.5_＋0.3） kg, irrespective of genders, were used in this study. Permanent magnet-16 was purchased from Shenyang NdFeB Permanent Magnetic Materials Co., Ltd. METHODS： The experimental animals, magnets, button and iron equipment were used to establish animal model systems concerned about the magnetic pull on bone growth. Twenty-four rabbits were divided into three groups, each group contained eight animals. Traction group used magnetic pull system and were kept in the iron cage. Magnetic group was fixed only in the foot plate of rabbit right legs using magnetic disk, rabbits were also fed in the iron cage. Control group received no ordinary device and only fed in bamboo cage. MAIN OUTCOME MEASURES： Four months later, the femoral and tibial growth of all rabbits were determined. . RESULTS： Rabbits were treated separately for 4 months. Compared with the control group, the tibial bone grew 2.61 mm and femoral bone grew 1.56 mm in the treated group. In the magnetic group, tibial growth increased 0.728 mm and femoral growth increased 0.41 mm, the differences were significant （P 〈 0.01）. CONCLUSION： ＂Model system＂ in the control experiments can simulate and achieve a state of magnetic pulling of the entire process at natural movement, and it is able to meet the requirement of the study on the effect of pulling traction on skeletal growth due to low cost, easy operation and strong repeatability.